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Purposeexpression of EGFP tagged Cdc42BPB
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50759 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCdc42BPB
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Alt nameCdc42 binding protein kinase beta
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SpeciesH. sapiens (human)
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Entrez GeneCDC42BPB (a.k.a. MRCKB)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1-Cdc42BPB was a gift from Naoki Mochizuki (Addgene plasmid # 50759 ; http://n2t.net/addgene:50759 ; RRID:Addgene_50759) -
For your References section:
Rap1 potentiates endothelial cell junctions by spatially controlling myosin II activity and actin organization. Ando K, Fukuhara S, Moriya T, Obara Y, Nakahata N, Mochizuki N. J Cell Biol. 2013 Sep 16;202(6):901-16. doi: 10.1083/jcb.201301115. Epub 2013 Sep 9. 10.1083/jcb.201301115 PubMed 24019534