ClopHensorN
(Plasmid #50758)

• Purpose: Mammalian expression vector expressing the ClopHensorN chloride and proton sensor
• Depositing Lab: Colin Akerman
• Publication: Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

Backbone

• Vector backbone: pBJ1
• Backbone size w/o insert (bp): 6326
• Total vector size (bp): 8590
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: No problems with this plasmid. Can use standard strains for growth.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ClopHensorN
• Alt name: E2GFP fused to Tandem Tomato
• Species: Aq. Victoria and Discosoma sp
• Insert Size (bp): 2264
• Mutation: E2GFP is EGFP-T203Y
• Promoter: CAG
• Tag / Fusion Protein:
  • Strep-II (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pCAG_F_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This DNA plasmid incorporates genetic material from pcDNA3-ClopHensor (Plasmid 25938 on Addgene), which was supplied by: Dr. Daniele Arosio, Istituto di Biofisica, Consiglio Nazionale delle Ricerche & FBK, Via Sommarive, 18, I-38123 Trento, Italy.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry