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PurposeExpresses MagFRET-6 in the cytosol of mammalian cells. Weak affinity control FRET sensor for MagFRET-1.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50756 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegene encoding for MagFRET-6
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMVMagFRET-6 was a gift from Maarten Merkx (Addgene plasmid # 50756 ; http://n2t.net/addgene:50756 ; RRID:Addgene_50756) -
For your References section:
MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. Lindenburg LH, Vinkenborg JL, Oortwijn J, Aper SJ, Merkx M. PLoS One. 2013 Dec 2;8(12):e82009. doi: 10.1371/journal.pone.0082009. 10.1371/journal.pone.0082009 PubMed 24312622