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PurposeExpresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50742 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegene encoding for MagFRET-1
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMVMagFRET-1 was a gift from Maarten Merkx (Addgene plasmid # 50742 ; http://n2t.net/addgene:50742 ; RRID:Addgene_50742) -
For your References section:
MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. Lindenburg LH, Vinkenborg JL, Oortwijn J, Aper SJ, Merkx M. PLoS One. 2013 Dec 2;8(12):e82009. doi: 10.1371/journal.pone.0082009. 10.1371/journal.pone.0082009 PubMed 24312622
