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Addgene

pCDNA CrkII Y221F
(Plasmid #50732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA 3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CrkII Y221F
  • Alt name
    CrkII
  • Species
    H. sapiens (human)
  • Mutation
    Y221F
  • GenBank ID
    GI:1219554
  • Entrez Gene
    CRK (a.k.a. CRKII, p38)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA CrkII Y221F was a gift from Kenneth Yamada (Addgene plasmid # 50732 ; http://n2t.net/addgene:50732 ; RRID:Addgene_50732)

  • For your References section:

    Tyrosine phosphorylation of the CrkII adaptor protein modulates cell migration. Takino T, Tamura M, Miyamori H, Araki M, Matsumoto K, Sato H, Yamada KM. J Cell Sci. 2003 Aug 1;116(Pt 15):3145-55. Epub 2003 Jun 10. 10.1242/jcs.00632 PubMed 12799422
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