pCAG-EGxxFP Citations (57)
Originally described in: Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355. PubMed Journal
Articles Citing pCAG-EGxxFP
| Articles |
|---|
| CRISPR/Cas9-based genome editing in mice by single plasmid injection. Fujihara Y, Ikawa M. Methods Enzymol. 2014;546:319-36. doi: 10.1016/B978-0-12-801185-0.00015-5. PubMed |
| CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Kimura Y, Oda M, Nakatani T, Sekita Y, Monfort A, Wutz A, Mochizuki H, Nakano T. Sci Rep. 2015 Jun 3;5:10710. doi: 10.1038/srep10710. PubMed |
| Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Mizuno S, Takami K, Daitoku Y, Tanimoto Y, Dinh TT, Mizuno-Iijima S, Hasegawa Y, Takahashi S, Sugiyama F, Yagami K. Sci Rep. 2015 Sep 8;5:13632. doi: 10.1038/srep13632. PubMed |
| Involvement of FKBP6 in hepatitis C virus replication. Kasai H, Kawakami K, Yokoe H, Yoshimura K, Matsuda M, Yasumoto J, Maekawa S, Yamashita A, Tanaka T, Ikeda M, Kato N, Okamoto T, Matsuura Y, Sakamoto N, Enomoto N, Takeda S, Fujii H, Tsubuki M, Kusunoki M, Moriishi K. Sci Rep. 2015 Nov 16;5:16699. doi: 10.1038/srep16699. PubMed |
| Hepatocyte factor JMJD5 regulates HBV replication through interaction with HBx. Kouwaki T, Okamoto T, Ito A, Sugiyama Y, Yamashita K, Suzuki T, Kusakabe S, Hirano J, Fukuhara T, Yamashita A, Saito K, Okuzaki D, Watashi K, Sugiyama M, Yoshio S, Standley DM, Kanto T, Mizokami M, Moriishi K, Matsuura Y. J Virol. 2016 Jan 20. pii: JVI.02776-15. PubMed |
| Pathologic activation of thrombopoietin receptor and JAK2-STAT5 pathway by frameshift mutants of mouse calreticulin. Balligand T, Achouri Y, Pecquet C, Chachoua I, Nivarthi H, Marty C, Vainchenker W, Plo I, Kralovics R, Constantinescu SN. Leukemia. 2016 Feb 29. doi: 10.1038/leu.2016.47. PubMed |
| A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research. Miano JM, Zhu QM, Lowenstein CJ. Arterioscler Thromb Vasc Biol. 2016 Jun;36(6):1058-75. doi: 10.1161/ATVBAHA.116.304790. Epub 2016 Apr 21. PubMed |
| TRC8-dependent degradation of hepatitis C virus immature core protein regulates viral propagation and pathogenesis. Aizawa S, Okamoto T, Sugiyama Y, Kouwaki T, Ito A, Suzuki T, Ono C, Fukuhara T, Yamamoto M, Okochi M, Hiraga N, Imamura M, Chayama K, Suzuki R, Shoji I, Moriishi K, Moriya K, Koike K, Matsuura Y. Nat Commun. 2016 May 4;7:11379. doi: 10.1038/ncomms11379. PubMed |
Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells.
Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, Fodor E, Welker E.
Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
PubMed
Associated Plasmids |
| Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques. Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K. Sci Rep. 2016 Oct 26;6:35861. doi: 10.1038/srep35861. PubMed |
| CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. Young SA, Miyata H, Satouh Y, Aitken RJ, Baker MA, Ikawa M. J Cell Sci. 2016 Dec 1;129(23):4379-4387. Epub 2016 Oct 19. PubMed |
| Loss of maternal ANNEXIN A10 via a 34-kb deleted-type copy number variation is associated with embryonic mortality in Japanese Black cattle. Sasaki S, Ibi T, Akiyama T, Fukushima M, Sugimoto Y. BMC Genomics. 2016 Nov 24;17(1):968. PubMed |
| Delta40p53 suppresses tumor cell proliferation and induces cellular senescence in hepatocellular carcinoma cells. Ota A, Nakao H, Sawada Y, Karnan S, Wahiduzzaman M, Inoue T, Kobayashi Y, Yamamoto T, Ishii N, Ohashi T, Nakade Y, Sato K, Itoh K, Konishi H, Hosokawa Y, Yoneda M. J Cell Sci. 2016 Dec 15. pii: jcs.190736. PubMed |
| Inhibition of CRISPR/Cas9-Mediated Genome Engineering by a Type I Interferon-Induced Reduction in Guide RNA Expression. Machitani M, Sakurai F, Wakabayashi K, Nakatani K, Takayama K, Tachibana M, Mizuguchi H. Biol Pharm Bull. 2017;40(3):272-277. doi: 10.1248/bpb.b16-00700. PubMed |
| Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids. Noda T, Oji A, Ikawa M. Methods Mol Biol. 2017;1630:67-80. doi: 10.1007/978-1-4939-7128-2_6. PubMed |
| Hepatitis B virus prevents excessive viral production via reduction of cell death-inducing DFF45-like effectors. Yasumoto J, Kasai H, Yoshimura K, Otoguro T, Watashi K, Wakita T, Yamashita A, Tanaka T, Takeda S, Moriishi K. J Gen Virol. 2017 Jul;98(7):1762-1773. doi: 10.1099/jgv.0.000813. PubMed |
| Intein-mediated assembly of tunable scaffoldins for facile synthesis of designer cellulosomes. Han Z, Su WW. Appl Microbiol Biotechnol. 2018 Feb;102(3):1331-1342. doi: 10.1007/s00253-017-8701-y. Epub 2017 Dec 23. PubMed |
| Intravesicular Acidification Regulates Lipopolysaccharide Inflammation and Tolerance through TLR4 Trafficking. Murase M, Kawasaki T, Hakozaki R, Sueyoshi T, Putri DDP, Kitai Y, Sato S, Ikawa M, Kawai T. J Immunol. 2018 Apr 15;200(8):2798-2808. doi: 10.4049/jimmunol.1701390. Epub 2018 Mar 14. PubMed |
| Seamless Genetic Conversion of SMN2 to SMN1 via CRISPR/Cpf1 and Single-Stranded Oligodeoxynucleotides in Spinal Muscular Atrophy Patient-Specific Induced Pluripotent Stem Cells. Zhou M, Hu Z, Qiu L, Zhou T, Feng M, Hu Q, Zeng B, Li Z, Sun Q, Wu Y, Liu X, Wu L, Liang D. Hum Gene Ther. 2018 Nov;29(11):1252-1263. doi: 10.1089/hum.2017.255. Epub 2018 May 9. PubMed |
| Physiological function of phospholipase D2 in anti-tumor immunity: regulation of CD8(+) T lymphocyte proliferation. Ngo Thai Bich V, Hongu T, Miura Y, Katagiri N, Ohbayashi N, Yamashita-Kanemaru Y, Shibuya A, Funakoshi Y, Kanaho Y. Sci Rep. 2018 Apr 19;8(1):6283. doi: 10.1038/s41598-018-24512-x. PubMed |
| ZMYND10 functions in a chaperone relay during axonemal dynein assembly. Mali GR, Yeyati PL, Mizuno S, Dodd DO, Tennant PA, Keighren MA, Zur Lage P, Shoemark A, Garcia-Munoz A, Shimada A, Takeda H, Edlich F, Takahashi S, von Kreigsheim A, Jarman AP, Mill P. Elife. 2018 Jun 19;7. pii: 34389. doi: 10.7554/eLife.34389. PubMed |
| CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer. Feng W, Herbst L, Lichter P, Pfister SM, Liu HK, Kawauchi D. J Vis Exp. 2018 Jun 9;(136). doi: 10.3791/57311. PubMed |
| RSPH6A is required for sperm flagellum formation and male fertility in mice. Abbasi F, Miyata H, Shimada K, Morohoshi A, Nozawa K, Matsumura T, Xu Z, Pratiwi P, Ikawa M. J Cell Sci. 2018 Oct 11;131(19). pii: jcs.221648. doi: 10.1242/jcs.221648. PubMed |
| Infection with flaviviruses requires BCLXL for cell survival. Suzuki T, Okamoto T, Katoh H, Sugiyama Y, Kusakabe S, Tokunaga M, Hirano J, Miyata Y, Fukuhara T, Ikawa M, Satoh T, Yoshio S, Suzuki R, Saijo M, Huang DCS, Kanto T, Akira S, Matsuura Y. PLoS Pathog. 2018 Sep 27;14(9):e1007299. doi: 10.1371/journal.ppat.1007299. eCollection 2018 Sep. PubMed |
| Paired CRISPR/Cas9 Nickases Mediate Efficient Site-Specific Integration of F9 into rDNA Locus of Mouse ESCs. Wang Y, Zhao J, Duan N, Liu W, Zhang Y, Zhou M, Hu Z, Feng M, Liu X, Wu L, Li Z, Liang D. Int J Mol Sci. 2018 Oct 5;19(10). pii: ijms19103035. doi: 10.3390/ijms19103035. PubMed |
| USP15 Participates in Hepatitis C Virus Propagation through Regulation of Viral RNA Translation and Lipid Droplet Formation. Kusakabe S, Suzuki T, Sugiyama Y, Haga S, Horike K, Tokunaga M, Hirano J, Zhang H, Chen DV, Ishiga H, Komoda Y, Ono C, Fukuhara T, Yamamoto M, Ikawa M, Satoh T, Akira S, Tanaka T, Moriishi K, Fukai M, Taketomi A, Yoshio S, Kanto T, Suzuki T, Okamoto T, Matsuura Y. J Virol. 2019 Mar 5;93(6). pii: JVI.01708-18. doi: 10.1128/JVI.01708-18. Print 2019 Mar 15. PubMed |
| Reprogramming the antigen specificity of B cells using genome-editing technologies. Voss JE, Gonzalez-Martin A, Andrabi R, Fuller RP, Murrell B, McCoy LE, Porter K, Huang D, Li W, Sok D, Le K, Briney B, Chateau M, Rogers G, Hangartner L, Feeney AJ, Nemazee D, Cannon P, Burton DR. Elife. 2019 Jan 17;8. pii: 42995. doi: 10.7554/eLife.42995. PubMed |
| Oxytocin receptor knockout prairie voles generated by CRISPR/Cas9 editing show reduced preference for social novelty and exaggerated repetitive behaviors. Horie K, Inoue K, Suzuki S, Adachi S, Yada S, Hirayama T, Hidema S, Young LJ, Nishimori K. Horm Behav. 2019 May;111:60-69. doi: 10.1016/j.yhbeh.2018.10.011. Epub 2018 Nov 8. PubMed |
| Human Genomic Safe Harbors and the Suicide Gene-Based Safeguard System for iPSC-Based Cell Therapy. Kimura Y, Shofuda T, Higuchi Y, Nagamori I, Oda M, Nakamori M, Onodera M, Kanematsu D, Yamamoto A, Katsuma A, Suemizu H, Nakano T, Kanemura Y, Mochizuki H. Stem Cells Transl Med. 2019 Jul;8(7):627-638. doi: 10.1002/sctm.18-0039. Epub 2019 Mar 19. PubMed |
| CRISPR/Cas9-mediated in vivo gene editing reveals that neuronal 5-HT1A receptors in the dorsal raphe nucleus contribute to body temperature regulation in mice. Nishitani N, Ohmura Y, Nagayasu K, Shibui N, Kaneko S, Ohashi A, Yoshida T, Yamanaka A, Yoshioka M. Brain Res. 2019 Sep 15;1719:243-252. doi: 10.1016/j.brainres.2019.06.009. Epub 2019 Jun 10. PubMed |
| CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice. Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda JM, Kiyozumi D, Zhang Q, Larasati T, Young SAM, Kodani M, Huddleston CA, Robertson MJ, Coarfa C, Isotani A, Aitken RJ, Okabe M, Matzuk MM, Garcia TX, Ikawa M. Biol Reprod. 2019 Jun 14. pii: 5519226. doi: 10.1093/biolre/ioz103. PubMed |
| Induction of t(11;14) IgH enhancer/promoter-cyclin D1 gene translocation using CRISPR/Cas9. Tsuyama N, Abe Y, Yanagi A, Yanai Y, Sugai M, Katafuchi A, Kawamura F, Kamiya K, Sakai A. Oncol Lett. 2019 Jul;18(1):275-282. doi: 10.3892/ol.2019.10303. Epub 2019 May 2. PubMed |
| Extracellular ADP augments microglial inflammasome and NF-kappaB activation via the P2Y12 receptor. Suzuki T, Kohyama K, Moriyama K, Ozaki M, Hasegawa S, Ueno T, Saitoe M, Morio T, Hayashi M, Sakuma H. Eur J Immunol. 2020 Feb;50(2):205-219. doi: 10.1002/eji.201848013. Epub 2019 Oct 2. PubMed |
| Anti-tumor effects of an antagonistic mAb against the ASCT2 amino acid transporter on KRAS-mutated human colorectal cancer cells. Hara Y, Minami Y, Yoshimoto S, Hayashi N, Yamasaki A, Ueda S, Masuko K, Masuko T. Cancer Med. 2020 Jan;9(1):302-312. doi: 10.1002/cam4.2689. Epub 2019 Nov 10. PubMed |
| Non-invasive in vivo imaging of UCP1 expression in live mice via near-infrared fluorescent protein iRFP720. Fukuda A, Honda S, Fujioka N, Sekiguchi Y, Mizuno S, Miwa Y, Sugiyama F, Hayashi Y, Nishimura K, Hisatake K. PLoS One. 2019 Nov 15;14(11):e0225213. doi: 10.1371/journal.pone.0225213. eCollection 2019. PubMed |
| CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice. Abbasi F, Kodani M, Emori C, Kiyozumi D, Mori M, Fujihara Y, Ikawa M. Cells. 2020 Mar 28;9(4). pii: cells9040821. doi: 10.3390/cells9040821. PubMed |
| TBC1D24 regulates recycling of clathrin-independent cargo proteins mediated by tubular recycling endosomes. Kim Nguyen NT, Ohbayashi N, Kanaho Y, Funakoshi Y. Biochem Biophys Res Commun. 2020 Jul 12;528(1):220-226. doi: 10.1016/j.bbrc.2020.05.007. Epub 2020 May 29. PubMed |
| Tmprss12 is required for sperm motility and uterotubal junction migration in micedagger. Larasati T, Noda T, Fujihara Y, Shimada K, Tobita T, Yu Z, Matzuk MM, Ikawa M. Biol Reprod. 2020 Aug 4;103(2):254-263. doi: 10.1093/biolre/ioaa060. PubMed |
| CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproductiondagger. Sun J, Lu Y, Nozawa K, Xu Z, Morohoshi A, Castaneda JM, Noda T, Miyata H, Abbasi F, Shawki HH, Takahashi S, Devlin DJ, Yu Z, Matzuk RM, Garcia TX, Matzuk MM, Ikawa M. Biol Reprod. 2020 Aug 4;103(2):183-194. doi: 10.1093/biolre/ioaa083. PubMed |
| Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Ishibashi R, Abe K, Ido N, Kitano S, Miyachi H, Toyoshima F. Sci Rep. 2020 Aug 24;10(1):14120. doi: 10.1038/s41598-020-70804-6. PubMed |
| Sulfated glycans engage the Ang-Tie pathway to regulate vascular development. Griffin ME, Sorum AW, Miller GM, Goddard WA 3rd, Hsieh-Wilson LC. Nat Chem Biol. 2021 Feb;17(2):178-186. doi: 10.1038/s41589-020-00657-7. Epub 2020 Oct 5. PubMed |
| Genome editing in plants using CRISPR type I-D nuclease. Osakabe K, Wada N, Miyaji T, Murakami E, Marui K, Ueta R, Hashimoto R, Abe-Hara C, Kong B, Yano K, Osakabe Y. Commun Biol. 2020 Nov 6;3(1):648. doi: 10.1038/s42003-020-01366-6. PubMed |
| Genome editing in mammalian cells using the CRISPR type I-D nuclease. Osakabe K, Wada N, Murakami E, Miyashita N, Osakabe Y. Nucleic Acids Res. 2021 Jun 21;49(11):6347-6363. doi: 10.1093/nar/gkab348. PubMed |
Precise CAG repeat contraction in a Huntington's Disease mouse model is enabled by gene editing with SpCas9-NG.
Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki O, Ikawa M.
Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w.
PubMed
Associated Plasmids |
| Brain-wide Cas9-mediated cleavage of a gene causing familial Alzheimer's disease alleviates amyloid-related pathologies in mice. Duan Y, Ye T, Qu Z, Chen Y, Miranda A, Zhou X, Lok KC, Chen Y, Fu AKY, Gradinaru V, Ip NY. Nat Biomed Eng. 2022 Feb;6(2):168-180. doi: 10.1038/s41551-021-00759-0. Epub 2021 Jul 26. PubMed |
| ECHDC1 knockout mice accumulate ethyl-branched lipids and excrete abnormal intermediates of branched-chain fatty acid metabolism. Dewulf JP, Paquay S, Marbaix E, Achouri Y, Van Schaftingen E, Bommer GT. J Biol Chem. 2021 Oct;297(4):101083. doi: 10.1016/j.jbc.2021.101083. Epub 2021 Aug 19. PubMed |
| Transposable elements that have recently been mobile in the human genome. Autio MI, Bin Amin T, Perrin A, Wong JY, Foo RS, Prabhakar S. BMC Genomics. 2021 Nov 3;22(1):789. doi: 10.1186/s12864-021-08085-0. PubMed |
| An oncogenic splice variant of PDGFRalpha in adult glioblastoma as a therapeutic target for selective CDK4/6 inhibitors. Hamada T, Akahane T, Yokoyama S, Higa N, Kirishima M, Matsuo K, Shimokawa M, Yoshimoto K, Tanimoto A. Sci Rep. 2022 Jan 24;12(1):1275. doi: 10.1038/s41598-022-05391-9. PubMed |
| Gulo gene locus, a new gene editing locus for mammalian cells. Habu T, Ishikawa H, Kim J. Biotechnol J. 2022 Jul;17(7):e2100493. doi: 10.1002/biot.202100493. Epub 2022 Apr 19. PubMed |
| Erratic and blood vessel-guided migration of astrocyte progenitors in the cerebral cortex. Tabata H, Sasaki M, Agetsuma M, Sano H, Hirota Y, Miyajima M, Hayashi K, Honda T, Nishikawa M, Inaguma Y, Ito H, Takebayashi H, Ema M, Ikenaka K, Nabekura J, Nagata KI, Nakajima K. Nat Commun. 2022 Nov 2;13(1):6571. doi: 10.1038/s41467-022-34184-x. PubMed |
| Vitamin C transporter SVCT1 serves a physiological role as a urate importer: functional analyses and in vivo investigations. Toyoda Y, Miyata H, Uchida N, Morimoto K, Shigesawa R, Kassai H, Nakao K, Tomioka NH, Matsuo H, Ichida K, Hosoyamada M, Aiba A, Suzuki H, Takada T. Pflugers Arch. 2023 Apr;475(4):489-504. doi: 10.1007/s00424-023-02792-1. Epub 2023 Feb 7. PubMed |
| Functional glycoproteomics by integrated network assembly and partitioning. Griffin ME, Thompson JW, Xiao Y, Sweredoski MJ, Aksenfeld RB, Jensen EH, Koldobskaya Y, Schacht AL, Kim TD, Choudhry P, Lomenick B, Garbis SD, Moradian A, Hsieh-Wilson LC. bioRxiv. 2023 Jun 14:2023.06.13.541482. doi: 10.1101/2023.06.13.541482. Preprint. PubMed |
| Generation of a familial hypercholesterolemia model in non-human primate. Sato A, Tsukiyama T, Komeno M, Iwatani C, Tsuchiya H, Kawamoto I, Murase M, Nakagawa T, Itagaki I, Seita Y, Matsumoto S, Nakaya M, Shimizu A, Yamada A, Ema M, Ogita H. Sci Rep. 2023 Sep 20;13(1):15649. doi: 10.1038/s41598-023-42763-1. PubMed |
| Regional random mutagenesis driven by multiple sgRNAs and diverse on-target genome editing events to identify functionally important elements in non-coding regions. Morimoto K, Suzuki H, Kuno A, Daitoku Y, Tanimoto Y, Kato K, Murata K, Sugiyama F, Mizuno S. Open Biol. 2024 Apr;14(4):240007. doi: 10.1098/rsob.240007. Epub 2024 Apr 3. PubMed |
| Generation and characterization of cerebellar granule neurons specific knockout mice of Golli-MBP. Miyazaki H, Nishioka S, Yamanaka T, Abe M, Imamura Y, Miyasaka T, Kakuda N, Oohashi T, Shimogori T, Yamakawa K, Ikawa M, Nukina N. Transgenic Res. 2024 Jun;33(3):99-117. doi: 10.1007/s11248-024-00382-0. Epub 2024 Apr 29. PubMed |
| Severe cardiac and skeletal manifestations in DMD-edited microminipigs: an advanced surrogate for Duchenne muscular dystrophy. Otake M, Imamura M, Enya S, Kangawa A, Shibata M, Ozaki K, Kimura K, Ono E, Aoki Y. Commun Biol. 2024 May 3;7(1):523. doi: 10.1038/s42003-024-06222-5. PubMed |
| Generating an organ-deficient animal model using a multi-targeted CRISPR-Cas9 system. Lim JJ, Murata Y, Yuri S, Kitamuro K, Kawai T, Isotani A. Sci Rep. 2024 May 9;14(1):10636. doi: 10.1038/s41598-024-61167-3. PubMed |
If you have published an article using this material, please email us at [email protected] to have your article added to this page.