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pCW-Cas9 Citations (177)

Originally described in: Genetic screens in human cells using the CRISPR-Cas9 system.
Wang T, Wei JJ, Sabatini DM, Lander ES Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. PubMed Journal

Articles Citing pCW-Cas9

Articles
Efficient Gene Disruption in Cultured Primary Human Endothelial Cells by CRISPR/Cas9. Abrahimi P, Chang WG, Kluger MS, Qyang Y, Tellides G, Saltzman WM, Pober JS. Circ Res. 2015 Jul 3;117(2):121-8. doi: 10.1161/CIRCRESAHA.117.306290. Epub 2015 May 4. PubMed
miR-663 Suppresses Oncogenic Function of CXCR4 in Glioblastoma. Shi Y, Chen C, Yu SZ, Liu Q, Rao J, Zhang HR, Xiao HL, Fu TW, Long H, He ZC, Zhou K, Yao XH, Cui YH, Zhang X, Ping YF, Bian XW. Clin Cancer Res. 2015 May 28. PubMed
The C-terminal Domain (CTD) of Human DNA Glycosylase NEIL1 Is Required for Forming BERosome Repair Complex with DNA Replication Proteins at the Replicating Genome: Dominant Negative Function of CTD. Hegde PM, Dutta A, Sengupta S, Mitra J, Adhikari S, Tomkinson AE, Li GM, Boldogh I, Hazra TK, Mitra S, Hegde ML. J Biol Chem. 2015 Jul 1. pii: jbc.M115.642918. PubMed
CRISPR/Cas9 System as an Agent for Eliminating Polyomavirus JC Infection. Wollebo HS, Bellizzi A, Kaminski R, Hu W, White MK, Khalili K. PLoS One. 2015 Sep 11;10(9):e0136046. doi: 10.1371/journal.pone.0136046. eCollection 2015. PubMed
AMPK Protects Leukemia-Initiating Cells in Myeloid Leukemias from Metabolic Stress in the Bone Marrow. Saito Y, Chapple RH, Lin A, Kitano A, Nakada D. Cell Stem Cell. 2015 Sep 29. pii: S1934-5909(15)00374-4. doi: 10.1016/j.stem.2015.08.019. PubMed

Associated Plasmids
Genome editing through large insertion leads to the skipping of targeted exon. Uddin B, Chen NP, Panic M, Schiebel E. BMC Genomics. 2015 Dec 21;16(1):1082. doi: 10.1186/s12864-015-2284-8. PubMed
Fan1 deficiency results in DNA interstrand cross-link repair defects, enhanced tissue karyomegaly, and organ dysfunction. Thongthip S, Bellani M, Gregg SQ, Sridhar S, Conti BA, Chen Y, Seidman MM, Smogorzewska A. Genes Dev. 2016 Mar 15;30(6):645-59. doi: 10.1101/gad.276261.115. PubMed
BIN1 regulates BACE1 intracellular trafficking and amyloid-beta production. Miyagawa T, Ebinuma I, Morohashi Y, Hori Y, Young Chang M, Hattori H, Maehara T, Yokoshima S, Fukuyama T, Tsuji S, Iwatsubo T, Prendergast GC, Tomita T. Hum Mol Genet. 2016 Jul 15;25(14):2948-2958. Epub 2016 May 14. PubMed
Focal Adhesion Kinase regulates the DNA damage response and its inhibition radiosensitizes mutant KRAS lung cancer. Tang KJ, Constanzo J, Venkateswaran N, Melegari M, Ilcheva M, Morales JC, Skoulidis F, Heymach JV, Boothman DA, Scaglioni PP. Clin Cancer Res. 2016 May 24. pii: clincanres.2603.2015. PubMed
Generation of porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene by somatic cell nuclear transfer. Liu G, Liu K, Wei H, Li L, Zhang S. Mol Med Rep. 2016 Sep;14(3):2527-33. doi: 10.3892/mmr.2016.5530. Epub 2016 Jul 19. PubMed
Hypersensitivity to DNA damage in antephase as a safeguard for genome stability. Feringa FM, Krenning L, Koch A, van den Berg J, van den Broek B, Jalink K, Medema RH. Nat Commun. 2016 Aug 26;7:12618. doi: 10.1038/ncomms12618. PubMed
A PGC1alpha-mediated transcriptional axis suppresses melanoma metastasis. Luo C, Lim JH, Lee Y, Granter SR, Thomas A, Vazquez F, Widlund HR, Puigserver P. Nature. 2016 Sep 15;537(7620):422-426. doi: 10.1038/nature19347. Epub 2016 Aug 31. PubMed
Systematic Analysis of Known and Candidate Lysine Demethylases in the Regulation of Myoblast Differentiation. Munehira Y, Yang Z, Gozani O. J Mol Biol. 2016 Oct 11. pii: S0022-2836(16)30419-3. doi: 10.1016/j.jmb.2016.10.004. PubMed
A pH- and ionic strength-dependent conformational change in the neck region regulates DNGR-1 function in dendritic cells. Hanc P, Schulz O, Fischbach H, Martin SR, Kjaer S, Reis E Sousa C. EMBO J. 2016 Nov 15;35(22):2484-2497. Epub 2016 Oct 17. PubMed
Deficiency of XLF and PAXX prevents DNA double-strand break repair by non-homologous end joining in lymphocytes. Hung PJ, Chen BR, George R, Liberman C, Morales AJ, Colon-Ortiz P, Tyler JK, Sleckman BP, Bredemeyer AL. Cell Cycle. 2017 Feb;16(3):286-295. doi: 10.1080/15384101.2016.1253640. Epub 2016 Nov 10. PubMed
A High-Throughput Strategy for Dissecting Mammalian Genetic Interactions. Stockman VB, Ghamsari L, Lasso G, Honig B, Shapira SD, Wang HH. PLoS One. 2016 Dec 9;11(12):e0167617. doi: 10.1371/journal.pone.0167617. eCollection 2016. PubMed
Mechanism of PKR Inhibition by Human Cytomegalovirus pTRS1. Vincent HA, Ziehr B, Moorman NJ. J Virol. 2016 Dec 14. pii: JVI.01574-16. PubMed
MicroRNA-202 maintains spermatogonial stem cells by inhibiting cell cycle regulators and RNA binding proteins. Chen J, Cai T, Zheng C, Lin X, Wang G, Liao S, Wang X, Gan H, Zhang D, Hu X, Wang S, Li Z, Feng Y, Yang F, Han C. Nucleic Acids Res. 2016 Dec 19. pii: gkw1287. doi: 10.1093/nar/gkw1287. PubMed
Application of CRISPR/Cas9 to Autophagy Research. O'Prey J, Sakamaki J, Baudot AD, New M, Van Acker T, Tooze SA, Long JS, Ryan KM. Methods Enzymol. 2017;588:79-108. doi: 10.1016/bs.mie.2016.09.076. Epub 2016 Dec 1. PubMed
ENL links histone acetylation to oncogenic gene expression in acute myeloid leukaemia. Wan L, Wen H, Li Y, Lyu J, Xi Y, Hoshii T, Joseph JK, Wang X, Loh YE, Erb MA, Souza AL, Bradner JE, Shen L, Li W, Li H, Allis CD, Armstrong SA, Shi X. Nature. 2017 Mar 9;543(7644):265-269. doi: 10.1038/nature21687. Epub 2017 Mar 1. PubMed
CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Xia L, Huang W, Bellani M, Seidman MM, Wu K, Fan D, Nie Y, Cai Y, Zhang YW, Yu LR, Li H, Zahnow CA, Xie W, Chiu Yen RW, Rassool FV, Baylin SB. Cancer Cell. 2017 May 8;31(5):653-668.e7. doi: 10.1016/j.ccell.2017.04.005. PubMed
MLL2, Not MLL1, Plays a Major Role in Sustaining MLL-Rearranged Acute Myeloid Leukemia. Chen Y, Anastassiadis K, Kranz A, Stewart AF, Arndt K, Waskow C, Yokoyama A, Jones K, Neff T, Lee Y, Ernst P. Cancer Cell. 2017 Jun 12;31(6):755-770.e6. doi: 10.1016/j.ccell.2017.05.002. PubMed
Chromatin-Bound Oxidized alpha-Synuclein Causes Strand Breaks in Neuronal Genomes in in vitro Models of Parkinson's Disease. Vasquez V, Mitra J, Hegde PM, Pandey A, Sengupta S, Mitra S, Rao KS, Hegde ML. J Alzheimers Dis. 2017;60(s1):S133-S150. doi: 10.3233/JAD-170342. PubMed
Smac mimetics and oncolytic viruses synergize in driving anticancer T-cell responses through complementary mechanisms. Kim DS, Dastidar H, Zhang C, Zemp FJ, Lau K, Ernst M, Rakic A, Sikdar S, Rajwani J, Naumenko V, Balce DR, Ewanchuk BW, Tailor P, Yates RM, Jenne C, Gafuik C, Mahoney DJ. Nat Commun. 2017 Aug 24;8(1):344. doi: 10.1038/s41467-017-00324-x. PubMed
RUNX2 expression in thyroid and breast cancer requires the cooperation of three non-redundant enhancers under the control of BRD4 and c-JUN. Sancisi V, Manzotti G, Gugnoni M, Rossi T, Gandolfi G, Gobbi G, Torricelli F, Catellani F, Faria do Valle I, Remondini D, Castellani G, Ragazzi M, Piana S, Ciarrocchi A. Nucleic Acids Res. 2017 Nov 2;45(19):11249-11267. doi: 10.1093/nar/gkx802. PubMed
CRISPR-Cas9-mediated functional dissection of 3'-UTRs. Zhao W, Siegel D, Biton A, Tonqueze OL, Zaitlen N, Ahituv N, Erle DJ. Nucleic Acids Res. 2017 Oct 13;45(18):10800-10810. doi: 10.1093/nar/gkx675. PubMed
Ebola virus requires phosphatidylinositol (3,5) bisphosphate production for efficient viral entry. Qiu S, Leung A, Bo Y, Kozak RA, Anand SP, Warkentin C, Salambanga FDR, Cui J, Kobinger G, Kobasa D, Cote M. Virology. 2018 Jan 1;513:17-28. doi: 10.1016/j.virol.2017.09.028. Epub 2017 Oct 12. PubMed
Antagonism of the Protein Kinase R Pathway in Human Cells by Rhesus Cytomegalovirus. Child SJ, Hickson SE, Bayer A, Malouli D, Fruh K, Geballe AP. J Virol. 2018 Feb 26;92(6). pii: JVI.01793-17. doi: 10.1128/JVI.01793-17. Print 2018 Mar 15. PubMed
On the design of CRISPR-based single-cell molecular screens. Hill AJ, McFaline-Figueroa JL, Starita LM, Gasperini MJ, Matreyek KA, Packer J, Jackson D, Shendure J, Trapnell C. Nat Methods. 2018 Feb 19. pii: nmeth.4604. doi: 10.1038/nmeth.4604. PubMed

Associated Plasmids
A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Hoshii T, Cifani P, Feng Z, Huang CH, Koche R, Chen CW, Delaney CD, Lowe SW, Kentsis A, Armstrong SA. Cell. 2018 Feb 22;172(5):1007-1021.e17. doi: 10.1016/j.cell.2018.01.032. PubMed
CAMKs support development of acute myeloid leukemia. Kang X, Cui C, Wang C, Wu G, Chen H, Lu Z, Chen X, Wang L, Huang J, Geng H, Zhao M, Chen Z, Muschen M, Wang HY, Zhang CC. J Hematol Oncol. 2018 Feb 27;11(1):30. doi: 10.1186/s13045-018-0574-8. PubMed
MicroRNA-focused CRISPR-Cas9 Library Screen Reveals Fitness-Associated miRNAs. Kurata JS, Lin RJ. RNA. 2018 May 2. pii: rna.066282.118. doi: 10.1261/rna.066282.118. PubMed

Associated Plasmids
Vitamin D Switches BAF Complexes to Protect beta Cells. Wei Z, Yoshihara E, He N, Hah N, Fan W, Pinto AFM, Huddy T, Wang Y, Ross B, Estepa G, Dai Y, Ding N, Sherman MH, Fang S, Zhao X, Liddle C, Atkins AR, Yu RT, Downes M, Evans RM. Cell. 2018 May 17;173(5):1135-1149.e15. doi: 10.1016/j.cell.2018.04.013. Epub 2018 May 10. PubMed
Oncogenic Properties of NEAT1 in Prostate Cancer Cells Depend on the CDC5L-AGRN Transcriptional Regulation Circuit. Li X, Wang X, Song W, Xu H, Huang R, Wang Y, Zhao W, Xiao Z, Yang X. Cancer Res. 2018 Aug 1;78(15):4138-4149. doi: 10.1158/0008-5472.CAN-18-0688. Epub 2018 Jun 5. PubMed
Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1. Magliozzi R, Carrero ZI, Low TY, Yuniati L, Valdes-Quezada C, Kruiswijk F, van Wijk K, Heck AJR, Jackson CL, Guardavaccaro D. Cell Rep. 2018 Jun 12;23(11):3381-3391.e4. doi: 10.1016/j.celrep.2018.05.031. PubMed
A multiprotein supercomplex controlling oncogenic signalling in lymphoma. Phelan JD, Young RM, Webster DE, Roulland S, Wright GW, Kasbekar M, Shaffer AL 3rd, Ceribelli M, Wang JQ, Schmitz R, Nakagawa M, Bachy E, Huang DW, Ji Y, Chen L, Yang Y, Zhao H, Yu X, Xu W, Palisoc MM, Valadez RR, Davies-Hill T, Wilson WH, Chan WC, Jaffe ES, Gascoyne RD, Campo E, Rosenwald A, Ott G, Delabie J, Rimsza LM, Rodriguez FJ, Estephan F, Holdhoff M, Kruhlak MJ, Hewitt SM, Thomas CJ, Pittaluga S, Oellerich T, Staudt LM. Nature. 2018 Aug;560(7718):387-391. doi: 10.1038/s41586-018-0290-0. Epub 2018 Jun 20. PubMed
CK1alpha and IRF4 are essential and independent effectors of immunomodulatory drugs in primary effusion lymphoma. Patil A, Manzano M, Gottwein E. Blood. 2018 Aug 9;132(6):577-586. doi: 10.1182/blood-2018-01-828418. Epub 2018 Jun 28. PubMed

Associated Plasmids
MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining. Hung PJ, Johnson B, Chen BR, Byrum AK, Bredemeyer AL, Yewdell WT, Johnson TE, Lee BJ, Deivasigamani S, Hindi I, Amatya P, Gross ML, Paull TT, Pisapia DJ, Chaudhuri J, Petrini JJH, Mosammaparast N, Amarasinghe GK, Zha S, Tyler JK, Sleckman BP. Mol Cell. 2018 Jul 19;71(2):332-342.e8. doi: 10.1016/j.molcel.2018.06.018. Epub 2018 Jul 12. PubMed
MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD. Cichewicz MA, Kiran M, Przanowska RK, Sobierajska E, Shibata Y, Dutta A. Mol Cell Biol. 2018 Sep 28;38(20). pii: MCB.00655-17. doi: 10.1128/MCB.00655-17. Print 2018 Oct 15. PubMed
PARP2 mediates branched poly ADP-ribosylation in response to DNA damage. Chen Q, Kassab MA, Dantzer F, Yu X. Nat Commun. 2018 Aug 13;9(1):3233. doi: 10.1038/s41467-018-05588-5. PubMed
Function of HNRNPC in breast cancer cells by controlling the dsRNA-induced interferon response. Wu Y, Zhao W, Liu Y, Tan X, Li X, Zou Q, Xiao Z, Xu H, Wang Y, Yang X. EMBO J. 2018 Dec 3;37(23). pii: embj.201899017. doi: 10.15252/embj.201899017. Epub 2018 Aug 29. PubMed
A limited number of double-strand DNA breaks is sufficient to delay cell cycle progression. van den Berg J, G Manjon A, Kielbassa K, Feringa FM, Freire R, Medema RH. Nucleic Acids Res. 2018 Nov 2;46(19):10132-10144. doi: 10.1093/nar/gky786. PubMed
PLK1 targets CtIP to promote microhomology-mediated end joining. Wang H, Qiu Z, Liu B, Wu Y, Ren J, Liu Y, Zhao Y, Wang Y, Hao S, Li Z, Peng B, Xu X. Nucleic Acids Res. 2018 Nov 16;46(20):10724-10739. doi: 10.1093/nar/gky810. PubMed
Unscheduled HDAC4 repressive activity in human fibroblasts triggers TP53-dependent senescence and favors cell transformation. Paluvai H, Di Giorgio E, Brancolini C. Mol Oncol. 2018 Dec;12(12):2165-2181. doi: 10.1002/1878-0261.12392. Epub 2018 Nov 14. PubMed
An Inducible Alpha-Synuclein Expressing Neuronal Cell Line Model for Parkinson's Disease1. Vasquez V, Mitra J, Perry G, Rao KS, Hegde ML. J Alzheimers Dis. 2018;66(2):453-460. doi: 10.3233/JAD-180610. PubMed
SHQ1 regulation of RNA splicing is required for T-lymphoblastic leukemia cell survival. Su H, Hu J, Huang L, Yang Y, Thenoz M, Kuchmiy A, Hu Y, Li P, Feng H, Zhou Y, Taghon T, Van Vlierberghe P, Qing G, Chen Z, Liu H. Nat Commun. 2018 Oct 15;9(1):4281. doi: 10.1038/s41467-018-06523-4. PubMed
LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, He L, Chen Y, Chen H, Luo W, Lu Z, Xie J, Churchill H, Xu Y, Zhou Z, Wu G, Yu C, John S, Hirayasu K, Nguyen N, Liu X, Huang F, Li L, Deng H, Tang H, Sadek AH, Zhang L, Huang T, Zou Y, Chen B, Zhu H, Arase H, Xia N, Jiang Y, Collins R, You MJ, Homsi J, Unni N, Lewis C, Chen GQ, Fu YX, Liao XC, An Z, Zheng J, Zhang N, Zhang CC. Nature. 2018 Oct;562(7728):605-609. doi: 10.1038/s41586-018-0615-z. Epub 2018 Oct 17. PubMed
Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors. Bulatov E, Sayarova R, Mingaleeva R, Miftakhova R, Gomzikova M, Ignatyev Y, Petukhov A, Davidovich P, Rizvanov A, Barlev NA. Cell Death Discov. 2018 Nov 13;4:103. doi: 10.1038/s41420-018-0120-z. eCollection 2018. PubMed
Histone demethylase LSD1 is required for germinal center formation and BCL6-driven lymphomagenesis. Hatzi K, Geng H, Doane AS, Meydan C, LaRiviere R, Cardenas M, Duy C, Shen H, Vidal MNC, Baslan T, Mohammad HP, Kruger RG, Shaknovich R, Haberman AM, Inghirami G, Lowe SW, Melnick AM. Nat Immunol. 2019 Jan;20(1):86-96. doi: 10.1038/s41590-018-0273-1. Epub 2018 Dec 10. PubMed
The circadian E3 ligase complex SCF(FBXL3+CRY) targets TLK2. Correia SP, Chan AB, Vaughan M, Zolboot N, Perea V, Huber AL, Kriebs A, Moresco JJ, Yates JR 3rd, Lamia KA. Sci Rep. 2019 Jan 17;9(1):198. doi: 10.1038/s41598-018-36618-3. PubMed
Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays. Deshpande A, Chen BR, Zhao L, Saddoris K, Kerr M, Zhu N, Mali P, Deshpande AJ. J Vis Exp. 2019 Jan 7;(143). doi: 10.3791/58710. PubMed
Uncoupling protein 2 reprograms the tumor microenvironment to support the anti-tumor immune cycle. Cheng WC, Tsui YC, Ragusa S, Koelzer VH, Mina M, Franco F, Laubli H, Tschumi B, Speiser D, Romero P, Zippelius A, Petrova TV, Mertz K, Ciriello G, Ho PC. Nat Immunol. 2019 Feb;20(2):206-217. doi: 10.1038/s41590-018-0290-0. Epub 2019 Jan 21. PubMed
Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines. Webster DE, Roulland S, Phelan JD. Methods Mol Biol. 2019;1956:337-350. doi: 10.1007/978-1-4939-9151-8_16. PubMed
A KLF6-driven transcriptional network links lipid homeostasis and tumour growth in renal carcinoma. Syafruddin SE, Rodrigues P, Vojtasova E, Patel SA, Zaini MN, Burge J, Warren AY, Stewart GD, Eisen T, Bihary D, Samarajiwa SA, Vanharanta S. Nat Commun. 2019 Mar 11;10(1):1152. doi: 10.1038/s41467-019-09116-x. PubMed
Artemisinin compounds sensitize cancer cells to ferroptosis by regulating iron homeostasis. Chen GQ, Benthani FA, Wu J, Liang D, Bian ZX, Jiang X. Cell Death Differ. 2019 May 21. pii: 10.1038/s41418-019-0352-3. doi: 10.1038/s41418-019-0352-3. PubMed
Comprehensive substrate specificity profiling of the human Nek kinome reveals unexpected signaling outputs. van de Kooij B, Creixell P, van Vlimmeren A, Joughin BA, Miller CJ, Haider N, Simpson CD, Linding R, Stambolic V, Turk BE, Yaffe MB. Elife. 2019 May 24;8. pii: 44635. doi: 10.7554/eLife.44635. PubMed
Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. Artegiani B, van Voorthuijsen L, Lindeboom RGH, Seinstra D, Heo I, Tapia P, Lopez-Iglesias C, Postrach D, Dayton T, Oka R, Hu H, van Boxtel R, van Es JH, Offerhaus J, Peters PJ, van Rheenen J, Vermeulen M, Clevers H. Cell Stem Cell. 2019 Jun 6;24(6):927-943.e6. doi: 10.1016/j.stem.2019.04.017. Epub 2019 May 23. PubMed
FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes. Adams EJ, Karthaus WR, Hoover E, Liu D, Gruet A, Zhang Z, Cho H, DiLoreto R, Chhangawala S, Liu Y, Watson PA, Davicioni E, Sboner A, Barbieri CE, Bose R, Leslie CS, Sawyers CL. Nature. 2019 Jul;571(7765):408-412. doi: 10.1038/s41586-019-1318-9. Epub 2019 Jun 26. PubMed
Reversible Regulation of Polyubiquitin Gene UBC via Modified Inducible CRISPR/Cas9 System. Han SW, Jung BK, Park SH, Ryu KY. Int J Mol Sci. 2019 Jun 28;20(13). pii: ijms20133168. doi: 10.3390/ijms20133168. PubMed
Poly (ADP) ribose glycohydrolase can be effectively targeted in pancreatic cancer. Jain A, Agostini LC, McCarthy GA, Chand SN, Ramirez A, Nevler A, Cozzitorto J, Schultz CW, Lowder CY, Smith KM, Waddell ID, Raitses-Gurevich M, Stossel C, Gorman YG, Atias D, Yeo CJ, Winter JM, Olive KP, Golan T, Pishvaian MJ, Ogilvie D, James DI, Jordan AM, Brody JR. Cancer Res. 2019 Jul 4. pii: 0008-5472.CAN-18-3645. doi: 10.1158/0008-5472.CAN-18-3645. PubMed
Intercellular interaction dictates cancer cell ferroptosis via NF2-YAP signalling. Wu J, Minikes AM, Gao M, Bian H, Li Y, Stockwell BR, Chen ZN, Jiang X. Nature. 2019 Aug;572(7769):402-406. doi: 10.1038/s41586-019-1426-6. Epub 2019 Jul 24. PubMed
The Barrier Molecules Junction Plakoglobin, Filaggrin, and Dystonin Play Roles in Melanoma Growth and Angiogenesis. Leick KM, Rodriguez AB, Melssen MM, Benamar M, Lindsay RS, Eki R, Du KP, Parlak M, Abbas T, Engelhard VH, Slingluff CL Jr. Ann Surg. 2019 Oct;270(4):712-722. doi: 10.1097/SLA.0000000000003522. PubMed
Ex vivo screen identifies CDK12 as a metastatic vulnerability in osteosarcoma. Bayles I, Krajewska M, Pontius WD, Saiakhova A, Morrow JJ, Bartels C, Lu J, Faber ZJ, Fedorov Y, Hong ES, Karnuta JM, Rubin B, Adams DJ, George RE, Scacheri PC. J Clin Invest. 2019 Oct 1;129(10):4377-4392. doi: 10.1172/JCI127718. PubMed
IFN-gamma-induced PD-L1 expression in melanoma depends on p53 expression. Thiem A, Hesbacher S, Kneitz H, di Primio T, Heppt MV, Hermanns HM, Goebeler M, Meierjohann S, Houben R, Schrama D. J Exp Clin Cancer Res. 2019 Sep 11;38(1):397. doi: 10.1186/s13046-019-1403-9. PubMed
Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma. Meyer SN, Scuoppo C, Vlasevska S, Bal E, Holmes AB, Holloman M, Garcia-Ibanez L, Nataraj S, Duval R, Vantrimpont T, Basso K, Brooks N, Dalla-Favera R, Pasqualucci L. Immunity. 2019 Sep 17;51(3):535-547.e9. doi: 10.1016/j.immuni.2019.08.006. Epub 2019 Sep 10. PubMed
Progenitor-derived human endothelial cells evade alloimmunity by CRISPR/Cas9-mediated complete ablation of MHC expression. Merola J, Reschke M, Pierce RW, Qin L, Spindler S, Baltazar T, Manes TD, Lopez-Giraldez F, Li G, Bracaglia LG, Xie C, Kirkiles-Smith N, Saltzman WM, Tietjen GT, Tellides G, Pober JS. JCI Insight. 2019 Oct 17;4(20). pii: 129739. doi: 10.1172/jci.insight.129739. PubMed
BAP1 regulates epigenetic switch from pluripotency to differentiation in developmental lineages giving rise to BAP1-mutant cancers. Kuznetsov JN, Aguero TH, Owens DA, Kurtenbach S, Field MG, Durante MA, Rodriguez DA, King ML, Harbour JW. Sci Adv. 2019 Sep 18;5(9):eaax1738. doi: 10.1126/sciadv.aax1738. eCollection 2019 Sep. PubMed
Regional Gene Repression by DNA Double-Strand Breaks in G1 Phase Cells. Purman CE, Collins PL, Porter SI, Saini A, Gupta H, Sleckman BP, Oltz EM. Mol Cell Biol. 2019 Nov 25;39(24). pii: MCB.00181-19. doi: 10.1128/MCB.00181-19. Print 2019 Dec 15. PubMed
The MTH1 inhibitor TH588 is a microtubule-modulating agent that eliminates cancer cells by activating the mitotic surveillance pathway. Gul N, Karlsson J, Tangemo C, Linsefors S, Tuyizere S, Perkins R, Ala C, Zou Z, Larsson E, Bergo MO, Lindahl P. Sci Rep. 2019 Oct 11;9(1):14667. doi: 10.1038/s41598-019-51205-w. PubMed
An improved platform for functional assessment of large protein libraries in mammalian cells. Matreyek KA, Stephany JJ, Chiasson MA, Hasle N, Fowler DM. Nucleic Acids Res. 2020 Jan 10;48(1):e1. doi: 10.1093/nar/gkz910. PubMed
Patient-Derived Head and Neck Cancer Organoids Recapitulate EGFR Expression Levels of Respective Tissues and Are Responsive to EGFR-Targeted Photodynamic Therapy. Driehuis E, Spelier S, Beltran Hernandez I, de Bree R, M Willems S, Clevers H, Oliveira S. J Clin Med. 2019 Nov 5;8(11). pii: jcm8111880. doi: 10.3390/jcm8111880. PubMed
IRG1 and Inducible Nitric Oxide Synthase Act Redundantly with Other Interferon-Gamma-Induced Factors To Restrict Intracellular Replication of Legionella pneumophila. Price JV, Russo D, Ji DX, Chavez RA, DiPeso L, Lee AY, Coers J, Vance RE. mBio. 2019 Nov 12;10(6). pii: mBio.02629-19. doi: 10.1128/mBio.02629-19. PubMed
CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci. Chylinski K, Hubmann M, Hanna RE, Yanchus C, Michlits G, Uijttewaal ECH, Doench J, Schramek D, Elling U. Nat Commun. 2019 Nov 29;10(1):5454. doi: 10.1038/s41467-019-13403-y. PubMed

Associated Plasmids
Induction of an Alternative mRNA 5' Leader Enhances Translation of the Ciliopathy Gene Inpp5e and Resistance to Oncolytic Virus Infection. Hoang HD, Graber TE, Jia JJ, Vaidya N, Gilchrist VH, Xiang X, Li W, Cowan KN, Gkogkas CG, Jaramillo M, Jafarnejad SM, Alain T. Cell Rep. 2019 Dec 17;29(12):4010-4023.e5. doi: 10.1016/j.celrep.2019.11.072. PubMed
Construction of an Inducible CRISPR/Cas9 System for CXCR4 Gene and Demonstration of its Effects on MKN-45 Cells. Peng Y, Yang T, Tang X, Chen F, Wang S. Cell Biochem Biophys. 2019 Dec 24. pii: 10.1007/s12013-019-00898-x. doi: 10.1007/s12013-019-00898-x. PubMed
A solid-phase transfection platform for arrayed CRISPR screens. Sercin O, Reither S, Roidos P, Ballin N, Palikyras S, Baginska A, Rein K, Llamazares M, Halavatyi A, Winter H, Muley T, Jurkowska RZ, Abdollahi A, Zenke FT, Neumann B, Mardin BR. Mol Syst Biol. 2019 Dec;15(12):e8983. doi: 10.15252/msb.20198983. PubMed
Establishment of A Reversibly Inducible Porcine Granulosa Cell Line. Bai Y, Zhu C, Feng M, Pan B, Zhang S, Zhan X, Chen H, Wang B, Li J. Cells. 2020 Jan 8;9(1). pii: cells9010156. doi: 10.3390/cells9010156. PubMed
West Nile virus capsid protein inhibits autophagy by AMP-activated protein kinase degradation in neurological disease development. Kobayashi S, Yoshii K, Phongphaew W, Muto M, Hirano M, Orba Y, Sawa H, Kariwa H. PLoS Pathog. 2020 Jan 23;16(1):e1008238. doi: 10.1371/journal.ppat.1008238. eCollection 2020 Jan. PubMed
Cellular context- and protein level-dependent interaction of pluripotency factor OCT4A with multiple octamer motifs of the same target gene. Zhou N, Zhang XB, Chen C, Chen XY, Kang B, He JQ, Gong GZ, Wang YJ, Zhou YW. Life Sci. 2020 May 1;248:117461. doi: 10.1016/j.lfs.2020.117461. Epub 2020 Feb 22. PubMed
Cryptochromes modulate E2F family transcription factors. Chan AB, Huber AL, Lamia KA. Sci Rep. 2020 Mar 5;10(1):4077. doi: 10.1038/s41598-020-61087-y. PubMed
Electron transport chain activity is a predictor and target for venetoclax sensitivity in multiple myeloma. Bajpai R, Sharma A, Achreja A, Edgar CL, Wei C, Siddiqa AA, Gupta VA, Matulis SM, McBrayer SK, Mittal A, Rupji M, Barwick BG, Lonial S, Nooka AK, Boise LH, Nagrath D, Shanmugam M. Nat Commun. 2020 Mar 6;11(1):1228. doi: 10.1038/s41467-020-15051-z. PubMed
Live cell imaging of single RNA molecules with fluorogenic Mango II arrays. Cawte AD, Unrau PJ, Rueda DS. Nat Commun. 2020 Mar 9;11(1):1283. doi: 10.1038/s41467-020-14932-7. PubMed

Associated Plasmids
Telomere length heterogeneity in ALT cells is maintained by PML-dependent localization of the BTR complex to telomeres. Loe TK, Li JSZ, Zhang Y, Azeroglu B, Boddy MN, Denchi EL. Genes Dev. 2020 May 1;34(9-10):650-662. doi: 10.1101/gad.333963.119. Epub 2020 Mar 26. PubMed
TIE2 Induces Breast Cancer Cell Dormancy and Inhibits the Development of Osteolytic Bone Metastases. Drescher F, Juarez P, Arellano DL, Serafin-Higuera N, Olvera-Rodriguez F, Jimenez S, Licea-Navarro AF, Fournier PG. Cancers (Basel). 2020 Apr 3;12(4). pii: cancers12040868. doi: 10.3390/cancers12040868. PubMed
Phenotype instability of hepatocyte-like cells produced by direct reprogramming of mesenchymal stromal cells. Orge ID, Gadd VL, Barouh JL, Rossi EA, Carvalho RH, Smith I, Allahdadi KJ, Paredes BD, Silva DN, Damasceno PKF, Sampaio GL, Forbes SJ, Soares MBP, Souza BSF. Stem Cell Res Ther. 2020 Apr 10;11(1):154. doi: 10.1186/s13287-020-01665-z. PubMed
miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro. Przanowska RK, Sobierajska E, Su Z, Jensen K, Przanowski P, Nagdas S, Kashatus JA, Kashatus DF, Bhatnagar S, Lukens JR, Dutta A. FASEB J. 2020 Jun;34(6):7687-7702. doi: 10.1096/fj.201902855RR. Epub 2020 Apr 11. PubMed
Identification of calcium and integrin-binding protein 1 as a novel regulator of production of amyloid beta peptide using CRISPR/Cas9-based screening system. Chiu YW, Hori Y, Ebinuma I, Sato H, Hara N, Ikeuchi T, Tomita T. FASEB J. 2020 Jun;34(6):7661-7674. doi: 10.1096/fj.201902966RR. Epub 2020 Apr 19. PubMed
Distinct roles of BRCA2 in replication fork protection in response to hydroxyurea and DNA interstrand cross-links. Rickman KA, Noonan RJ, Lach FP, Sridhar S, Wang AT, Abhyankar A, Huang A, Kelly M, Auerbach AD, Smogorzewska A. Genes Dev. 2020 Jun 1;34(11-12):832-846. doi: 10.1101/gad.336446.120. Epub 2020 Apr 30. PubMed
Targeting RLIP with CRISPR/Cas9 controls tumor growth. Singhal J, Chikara S, Horne D, Awasthi S, Salgia R, Singhal SS. Carcinogenesis. 2020 May 19. pii: 5840500. doi: 10.1093/carcin/bgaa048. PubMed
Tumor Cell Associated Hyaluronan-CD44 Signaling Promotes Pro-Tumor Inflammation in Breast Cancer. Witschen PM, Chaffee TS, Brady NJ, Huggins DN, Knutson TP, LaRue RS, Munro SA, Tiegs L, McCarthy JB, Nelson AC, Schwertfeger KL. Cancers (Basel). 2020 May 22;12(5). pii: cancers12051325. doi: 10.3390/cancers12051325. PubMed
Filoviruses Use the HOPS Complex and UVRAG To Traffic to Niemann-Pick C1 Compartments during Viral Entry. Bo Y, Qiu S, Mulloy RP, Cote M. J Virol. 2020 Jul 30;94(16). pii: JVI.01002-20. doi: 10.1128/JVI.01002-20. Print 2020 Jul 30. PubMed
Dysfunctional telomeres trigger cellular senescence mediated by cyclic GMP-AMP synthase. Abdisalaam S, Bhattacharya S, Mukherjee S, Sinha D, Srinivasan K, Zhu M, Akbay EA, Sadek HA, Shay JW, Asaithamby A. J Biol Chem. 2020 Aug 7;295(32):11144-11160. doi: 10.1074/jbc.RA120.012962. Epub 2020 Jun 15. PubMed
Dual Role of WISP1 in maintaining glioma stem cells and tumor-supportive macrophages in glioblastoma. Tao W, Chu C, Zhou W, Huang Z, Zhai K, Fang X, Huang Q, Zhang A, Wang X, Yu X, Huang H, Wu Q, Sloan AE, Yu JS, Li X, Stark GR, Rich JN, Bao S. Nat Commun. 2020 Jun 15;11(1):3015. doi: 10.1038/s41467-020-16827-z. PubMed
The c-Rel-c-Myc axis controls metabolism and proliferation of human T leukemia cells. Li X, Luo G, Li T, Sun H, Wang W, Eiler E, Goldsmith JR, Chen YH. Mol Immunol. 2020 Sep;125:115-122. doi: 10.1016/j.molimm.2020.06.029. Epub 2020 Jul 10. PubMed
PPT1 inhibition enhances the antitumor activity of anti-PD-1 antibody in melanoma. Sharma G, Ojha R, Noguera-Ortega E, Rebecca VW, Attanasio J, Liu S, Piao S, Lee JJ, Nicastri MC, Harper SL, Ronghe A, Jain V, Winkler JD, Speicher DW, Mastio J, Gimotty PA, Xu X, Wherry EJ, Gabrilovich DI, Amaravadi RK. JCI Insight. 2020 Sep 3;5(17). pii: 133225. doi: 10.1172/jci.insight.133225. PubMed
Knockout of the Transducin-Like Enhancer of Split 6 Gene Affects the Proliferation and Cell Cycle Process of Mouse Spermatogonia. Feng M, Bai Y, Chen Y, Wang K. Int J Mol Sci. 2020 Aug 13;21(16). pii: ijms21165827. doi: 10.3390/ijms21165827. PubMed
Kaposi's Sarcoma-Associated Herpesvirus Drives a Super-Enhancer-Mediated Survival Gene Expression Program in Primary Effusion Lymphoma. Manzano M, Gunther T, Ju H, Nicholas J, Bartom ET, Grundhoff A, Gottwein E. mBio. 2020 Aug 25;11(4). pii: mBio.01457-20. doi: 10.1128/mBio.01457-20. PubMed
Suppression of non-homologous end joining does not rescue DNA repair defects in Fanconi anemia patient cells. Thongthip S, Conti BA, Lach FP, Smogorzewska A. Cell Cycle. 2020 Oct;19(19):2553-2561. doi: 10.1080/15384101.2020.1810394. Epub 2020 Aug 30. PubMed
C11orf95-RELA reprograms 3D epigenome in supratentorial ependymoma. Zhu JJ, Jillette N, Li XN, Cheng AW, Lau CC. Acta Neuropathol. 2020 Dec;140(6):951-960. doi: 10.1007/s00401-020-02225-8. Epub 2020 Sep 9. PubMed
A polyclonal allelic expression assay for detecting regulatory effects of transcript variants. Brandt M, Gokden A, Ziosi M, Lappalainen T. Genome Med. 2020 Sep 11;12(1):79. doi: 10.1186/s13073-020-00777-8. PubMed
Prolonged fasting drives a program of metabolic inflammation in human adipose tissue. Fazeli PK, Zhang Y, O'Keefe J, Pesaresi T, Lun M, Lawney B, Steinhauser ML. Mol Metab. 2020 Sep 28;42:101082. doi: 10.1016/j.molmet.2020.101082. PubMed

Associated Plasmids
PLA2G6 guards placental trophoblasts against ferroptotic injury. Beharier O, Tyurin VA, Goff JP, Guerrero-Santoro J, Kajiwara K, Chu T, Tyurina YY, St Croix CM, Wallace CT, Parry S, Parks WT, Kagan VE, Sadovsky Y. Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27319-27328. doi: 10.1073/pnas.2009201117. Epub 2020 Oct 21. PubMed
The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1 mutant lung cancer. Kim J, Lee HM, Cai F, Ko B, Yang C, Lieu EL, Muhammad N, Rhyne S, Li K, Haloul M, Gu W, Faubert B, Kaushik AK, Cai L, Kasiri S, Marriam U, Nham K, Girard L, Wang H, Sun X, Kim J, Minna JD, Unsal-Kacmaz K, DeBerardinis RJ. Nat Metab. 2020 Dec;2(12):1401-1412. doi: 10.1038/s42255-020-00316-0. Epub 2020 Nov 30. PubMed
Chromothripsis drives the evolution of gene amplification in cancer. Shoshani O, Brunner SF, Yaeger R, Ly P, Nechemia-Arbely Y, Kim DH, Fang R, Castillon GA, Yu M, Li JSZ, Sun Y, Ellisman MH, Ren B, Campbell PJ, Cleveland DW. Nature. 2021 Mar;591(7848):137-141. doi: 10.1038/s41586-020-03064-z. Epub 2020 Dec 23. PubMed
Single-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery. Xu X, Crow M, Rice BR, Li F, Harris B, Liu L, Demesa-Arevalo E, Lu Z, Wang L, Fox N, Wang X, Drenkow J, Luo A, Char SN, Yang B, Sylvester AW, Gingeras TR, Schmitz RJ, Ware D, Lipka AE, Gillis J, Jackson D. Dev Cell. 2021 Feb 22;56(4):557-568.e6. doi: 10.1016/j.devcel.2020.12.015. Epub 2021 Jan 4. PubMed
Soluble adenylyl cyclase regulates the cytosolic NADH/NAD(+) redox state and the bioenergetic switch between glycolysis and oxidative phosphorylation. Chang JC, Go S, Gilglioni EH, Duijst S, Panneman DM, Rodenburg RJ, Li HL, Huang HL, Levin LR, Buck J, Verhoeven AJ, Oude Elferink RPJ. Biochim Biophys Acta Bioenerg. 2021 Apr 1;1862(4):148367. doi: 10.1016/j.bbabio.2020.148367. Epub 2021 Jan 5. PubMed
c-Rel Is a Myeloid Checkpoint for Cancer Immunotherapy. Li T, Li X, Zamani A, Wang W, Lee CN, Li M, Luo G, Eiler E, Sun H, Ghosh S, Jin J, Murali R, Ruan Q, Shi W, Chen YH. Nat Cancer. 2020 May;1(5):507-517. doi: 10.1038/s43018-020-0061-3. Epub 2020 May 18. PubMed
Sphingomyelin Biosynthesis Is Essential for Phagocytic Signaling during Mycobacterium tuberculosis Host Cell Entry. Niekamp P, Guzman G, Leier HC, Rashidfarrokhi A, Richina V, Pott F, Barisch C, Holthuis JCM, Tafesse FG. mBio. 2021 Jan 26;12(1). pii: mBio.03141-20. doi: 10.1128/mBio.03141-20. PubMed
Phospholipase iPLA2beta averts ferroptosis by eliminating a redox lipid death signal. Sun WY, Tyurin VA, Mikulska-Ruminska K, Shrivastava IH, Anthonymuthu TS, Zhai YJ, Pan MH, Gong HB, Lu DH, Sun J, Duan WJ, Korolev S, Abramov AY, Angelova PR, Miller I, Beharier O, Mao GW, Dar HH, Kapralov AA, Amoscato AA, Hastings TG, Greenamyre TJ, Chu CT, Sadovsky Y, Bahar I, Bayir H, Tyurina YY, He RR, Kagan VE. Nat Chem Biol. 2021 Apr;17(4):465-476. doi: 10.1038/s41589-020-00734-x. Epub 2021 Feb 4. PubMed
Optimized protocol for gene editing in adipocytes using CRISPR-Cas9 technology. Qiu Y, Ding Q. STAR Protoc. 2021 Jan 27;2(1):100307. doi: 10.1016/j.xpro.2021.100307. eCollection 2021 Mar 19. PubMed
Bioinformatics and Functional Analyses Implicate Potential Roles for EOGT and L-fringe in Pancreatic Cancers. Barua R, Mizuno K, Tashima Y, Ogawa M, Takeuchi H, Taguchi A, Okajima T. Molecules. 2021 Feb 7;26(4). pii: molecules26040882. doi: 10.3390/molecules26040882. PubMed
Venetoclax sensitivity in multiple myeloma is associated with B-cell gene expression. Gupta VA, Barwick BG, Matulis SM, Shirasaki R, Jaye DL, Keats JJ, Oberlton B, Joseph NS, Hofmeister CC, Heffner LT, Dhodapkar MV, Nooka AK, Lonial S, Mitsiades CS, Kaufman JL, Boise LH. Blood. 2021 Jul 1;137(26):3604-3615. doi: 10.1182/blood.2020007899. PubMed
Biophysical and biochemical properties of Deup1 self-assemblies: a potential driver for deuterosome formation during multiciliogenesis. Yamamoto S, Yabuki R, Kitagawa D. Biol Open. 2021 Mar 3;10(3). pii: 10/3/bio056432. doi: 10.1242/bio.056432. PubMed
Architectural control of metabolic plasticity in epithelial cancer cells. Al-Masri M, Paliotti K, Tran R, Halaoui R, Lelarge V, Chatterjee S, Wang LT, Moraes C, McCaffrey L. Commun Biol. 2021 Mar 19;4(1):371. doi: 10.1038/s42003-021-01899-4. PubMed
Disruption of the MSL complex inhibits tumour maintenance by exacerbating chromosomal instability. Monserrat J, Morales Torres C, Richardson L, Wilson TS, Patel H, Domart MC, Horswell S, Song OR, Jiang M, Crawford M, Bui M, Dalal Y, Scaffidi P. Nat Cell Biol. 2021 Apr;23(4):401-412. doi: 10.1038/s41556-021-00657-2. Epub 2021 Apr 9. PubMed
Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway. Ganga AK, Kennedy MC, Oguchi ME, Gray S, Oliver KE, Knight TA, De La Cruz EM, Homma Y, Fukuda M, Breslow DK. Curr Biol. 2021 Jul 12;31(13):2895-2905.e7. doi: 10.1016/j.cub.2021.04.075. Epub 2021 May 13. PubMed
Deletion of a pseudogene within a fragile site triggers the oncogenic expression of the mitotic CCSER1 gene. Santoliquido BM, Frenquelli M, Contadini C, Bestetti S, Gaviraghi M, Barbieri E, De Antoni A, Albarello L, Amabile A, Gardini A, Lombardo A, Doglioni C, Provero P, Soddu S, Cittaro D, Tonon G. Life Sci Alliance. 2021 Jun 29;4(8). pii: 4/8/e202101019. doi: 10.26508/lsa.202101019. Print 2021 Aug. PubMed
BTK inhibition sensitizes Acute Lymphoblastic Leukemia to asparaginase by suppressing the Amino Acid Response pathway. Butler M, van Ingen Schenau DS, Yu J, Jenni S, Dobay MP, Hagelaar R, Vervoort BMT, Tee TM, Hoff FW, Meijerink JP, Kornblau SM, Bornhauser B, Bourquin JP, Kuiper RP, van der Meer LT, van Leeuwen FN. Blood. 2021 Jul 19. pii: S0006-4971(21)01372-0. doi: 10.1182/blood.2021011787. PubMed
Deacetylation as a receptor-regulated direct activation switch for pannexin channels. Chiu YH, Medina CB, Doyle CA, Zhou M, Narahari AK, Sandilos JK, Gonye EC, Gao HY, Guo SY, Parlak M, Lorenz UM, Conrads TP, Desai BN, Ravichandran KS, Bayliss DA. Nat Commun. 2021 Jul 23;12(1):4482. doi: 10.1038/s41467-021-24825-y. PubMed
Molecular control of cell density-mediated exit to quiescence. Fan Y, Meyer T. Cell Rep. 2021 Jul 27;36(4):109436. doi: 10.1016/j.celrep.2021.109436. PubMed
A NSD3-targeted PROTAC suppresses NSD3 and cMyc oncogenic nodes in cancer cells. Xu C, Meng F, Park KS, Storey AJ, Gong W, Tsai YH, Gibson E, Byrum SD, Li D, Edmondson RD, Mackintosh SG, Vedadi M, Cai L, Tackett AJ, Kaniskan HU, Jin J, Wang GG. Cell Chem Biol. 2022 Mar 17;29(3):386-397.e9. doi: 10.1016/j.chembiol.2021.08.004. Epub 2021 Aug 31. PubMed
LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells. Chen BR, Wang Y, Tubbs A, Zong D, Fowler FC, Zolnerowich N, Wu W, Bennett A, Chen CC, Feng W, Nussenzweig A, Tyler JK, Sleckman BP. Elife. 2021 Sep 3;10. pii: 68466. doi: 10.7554/eLife.68466. PubMed
The RNF8 and RNF168 Ubiquitin Ligases Regulate Pro- and Anti-Resection Activities at Broken DNA Ends During Non-Homologous End Joining. Chen BR, Wang Y, Shen ZJ, Bennett A, Hindi I, Tyler JK, Sleckman BP. DNA Repair (Amst). 2021 Dec;108:103217. doi: 10.1016/j.dnarep.2021.103217. Epub 2021 Sep 1. PubMed
SMPDL3B Predicts Poor Prognosis and Contributes to Development of Acute Myeloid Leukemia. Qu H, Zhu Y. Front Mol Biosci. 2021 Aug 24;8:695601. doi: 10.3389/fmolb.2021.695601. eCollection 2021. PubMed
Supraphysiologic Testosterone Induces Ferroptosis and Activates Immune Pathways through Nucleophagy in Prostate Cancer. Kumar R, Mendonca J, Owoyemi O, Boyapati K, Thomas N, Kanacharoen S, Coffey M, Topiwala D, Gomes C, Ozbek B, Jones T, Rosen M, Dong L, Wiens S, Brennen WN, Isaacs JT, De Marzo AM, Markowski MC, Antonarakis ES, Qian DZ, Pienta KJ, Pardoll DM, Carducci MA, Denmeade SR, Kachhap SK. Cancer Res. 2021 Dec 1;81(23):5948-5962. doi: 10.1158/0008-5472.CAN-20-3607. Epub 2021 Oct 13. PubMed
Co-occurrence of BAP1 and SF3B1 mutations in uveal melanoma induces cellular senescence. Yu L, Zhou D, Zhang G, Ren Z, Luo X, Liu P, Plouffe SW, Meng Z, Moroishi T, Li Y, Zhang Y, Brown JH, Liu S, Guan KL. Mol Oncol. 2022 Feb;16(3):607-629. doi: 10.1002/1878-0261.13128. Epub 2021 Nov 12. PubMed
Intracellular Cholesterol Pools Regulate Oncogenic Signaling and Epigenetic Circuitries in Early T-cell Precursor Acute Lymphoblastic Leukemia. Rashkovan M, Albero R, Gianni F, Perez-Duran P, Miller HI, Mackey AL, Paietta EM, Tallman MS, Rowe JM, Litzow MR, Wiernik PH, Luger S, Sulis ML, Soni RK, Ferrando AA. Cancer Discov. 2022 Mar 1;12(3):856-871. doi: 10.1158/2159-8290.CD-21-0551. PubMed
Breast tissue regeneration is driven by cell-matrix interactions coordinating multi-lineage stem cell differentiation through DDR1. Rauner G, Jin DX, Miller DH, Gierahn TM, Li CM, Sokol ES, Feng YX, Mathis RA, Love JC, Gupta PB, Kuperwasser C. Nat Commun. 2021 Dec 10;12(1):7116. doi: 10.1038/s41467-021-27401-6. PubMed
GCN2 kinase activation by ATP-competitive kinase inhibitors. Tang CP, Clark O, Ferrarone JR, Campos C, Lalani AS, Chodera JD, Intlekofer AM, Elemento O, Mellinghoff IK. Nat Chem Biol. 2022 Feb;18(2):207-215. doi: 10.1038/s41589-021-00947-8. Epub 2021 Dec 23. PubMed
Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis. Chen Q, Ma K, Liu X, Chen SH, Li P, Yu Y, Leung AKL, Yu X. Cell Discov. 2022 Jan 18;8(1):3. doi: 10.1038/s41421-021-00355-1. PubMed
Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity. Fu R, He W, Dou J, Villarreal OD, Bedford E, Wang H, Hou C, Zhang L, Wang Y, Ma D, Chen Y, Gao X, Depken M, Xu H. Nat Commun. 2022 Jan 25;13(1):474. doi: 10.1038/s41467-022-28028-x. PubMed
KIX domain determines a selective tumor-promoting role for EP300 and its vulnerability in small cell lung cancer. Kim KB, Kabra A, Kim DW, Xue Y, Huang Y, Hou PC, Zhou Y, Miranda LJ, Park JI, Shi X, Bender TP, Bushweller JH, Park KS. Sci Adv. 2022 Feb 18;8(7):eabl4618. doi: 10.1126/sciadv.abl4618. Epub 2022 Feb 16. PubMed
A non-catalytic scaffolding activity of hexokinase 2 contributes to EMT and metastasis. Blaha CS, Ramakrishnan G, Jeon SM, Nogueira V, Rho H, Kang S, Bhaskar P, Terry AR, Aissa AF, Frolov MV, Patra KC, Brooks Robey R, Hay N. Nat Commun. 2022 Feb 16;13(1):899. doi: 10.1038/s41467-022-28440-3. PubMed
High-Throughput Drug Library Screening in Primary KMT2A-Rearranged Infant ALL Cells Favors the Identification of Drug Candidates That Activate P53 Signaling. Wander P, Arentsen-Peters STCJM, Vrenken KS, Pinhanos SM, Koopmans B, Dolman MEM, Jones L, Garrido Castro P, Schneider P, Kerstjens M, Molenaar JJ, Pieters R, Zwaan CM, Stam RW. Biomedicines. 2022 Mar 10;10(3). pii: biomedicines10030638. doi: 10.3390/biomedicines10030638. PubMed
LAMP2A regulates the loading of proteins into exosomes. Ferreira JV, da Rosa Soares A, Ramalho J, Maximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girao H, van Niel G, Pereira P. Sci Adv. 2022 Mar 25;8(12):eabm1140. doi: 10.1126/sciadv.abm1140. Epub 2022 Mar 25. PubMed
OVOL2 impairs RHO GTPase signaling to restrain mitosis and aggressiveness of Anaplastic Thyroid Cancer. Gugnoni M, Manzotti G, Vitale E, Sauta E, Torricelli F, Reggiani F, Pistoni M, Piana S, Ciarrocchi A. J Exp Clin Cancer Res. 2022 Mar 25;41(1):108. doi: 10.1186/s13046-022-02316-2. PubMed

Associated Plasmids
PDZ Proteins SCRIB and DLG1 Regulate Myeloma Cell Surface CD86 Expression, Growth, and Survival. Moser-Katz T, Gavile CM, Barwick BG, Lee KP, Boise LH. Mol Cancer Res. 2022 Jul 6;20(7):1122-1136. doi: 10.1158/1541-7786.MCR-21-0681. PubMed
MYCN mediates cysteine addiction and sensitizes neuroblastoma to ferroptosis. Alborzinia H, Florez AF, Kreth S, Bruckner LM, Yildiz U, Gartlgruber M, Odoni DI, Poschet G, Garbowicz K, Shao C, Klein C, Meier J, Zeisberger P, Nadler-Holly M, Ziehm M, Paul F, Burhenne J, Bell E, Shaikhkarami M, Wurth R, Stainczyk SA, Wecht EM, Kreth J, Buttner M, Ishaque N, Schlesner M, Nicke B, Stresemann C, Llamazares-Prada M, Reiling JH, Fischer M, Amit I, Selbach M, Herrmann C, Wolfl S, Henrich KO, Hofer T, Trumpp A, Westermann F. Nat Cancer. 2022 Apr;3(4):471-485. doi: 10.1038/s43018-022-00355-4. Epub 2022 Apr 28. PubMed
Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis. Carter RJ, Milani M, Beckett AJ, Liu S, Prior IA, Cohen GM, Varadarajan S. Cell Death Dis. 2022 May 4;13(5):436. doi: 10.1038/s41419-022-04869-8. PubMed
DNA-PK promotes DNA end resection at DNA double strand breaks in G(0) cells. Fowler FC, Chen BR, Zolnerowich N, Wu W, Pavani R, Paiano J, Peart C, Chen Z, Nussenzweig A, Sleckman BP, Tyler JK. Elife. 2022 May 16;11:e74700. doi: 10.7554/eLife.74700. PubMed
AURKA and PLK1 inhibition selectively and synergistically block cell cycle progression in diffuse midline glioma. Metselaar DS, du Chatinier A, Meel MH, Ter Huizen G, Waranecki P, Goulding JR, Bugiani M, Koster J, Kaspers GJL, Hulleman E. iScience. 2022 May 13;25(6):104398. doi: 10.1016/j.isci.2022.104398. eCollection 2022 Jun 17. PubMed
Double-strand break toxicity is chromatin context independent. Friskes A, Koob L, Krenning L, Severson TM, Koeleman ES, Vergara X, Schubert M, van den Berg J, Evers B, Manjon AG, Joosten S, Kim Y, Zwart W, Medema RH. Nucleic Acids Res. 2022 Sep 23;50(17):9930-9947. doi: 10.1093/nar/gkac758. PubMed
Unorthodox PCNA Binding by Chromatin Assembly Factor 1. Gopinathan Nair A, Rabas N, Lejon S, Homiski C, Osborne MJ, Cyr N, Sverzhinsky A, Melendy T, Pascal JM, Laue ED, Borden KLB, Omichinski JG, Verreault A. Int J Mol Sci. 2022 Sep 21;23(19):11099. doi: 10.3390/ijms231911099. PubMed
Chemical inhibition of DPP9 sensitizes the CARD8 inflammasome in HIV-1-infected cells. Clark KM, Kim JG, Wang Q, Gao H, Presti RM, Shan L. Nat Chem Biol. 2023 Apr;19(4):431-439. doi: 10.1038/s41589-022-01182-5. Epub 2022 Nov 10. PubMed
Exploration of the regulatory relationship between KRAB-Zfp clusters and their target transposable elements via a gene editing strategy at the cluster specific linker-associated sequences by CRISPR-Cas9. Zhang Y, He F, Zhang Y, Dai Q, Li Q, Nan J, Miao R, Cheng B. Mob DNA. 2022 Nov 10;13(1):25. doi: 10.1186/s13100-022-00279-x. PubMed
QSER1 preserves the suppressive status of the pro-apoptotic genes to prevent apoptosis. Zhao X, Fang K, Liu X, Yao R, Wang M, Li F, Hao S, He J, Wang Y, Fan M, Huang W, Li Y, Gao C, Lin C, Luo Z. Cell Death Differ. 2023 Mar;30(3):779-793. doi: 10.1038/s41418-022-01085-x. Epub 2022 Nov 12. PubMed
RAGE Ablation Attenuates Glioma Progression and Enhances Tumor Immune Responses by Suppressing Galectin-3 Expression. Zhang IY, Liu S, Zhang L, Liang R, Fang Q, Zhao J, Ren L, Medina EF, Filippov A, Bonjoc KJ, Chaudhry A, Dayyani M, Bild AH, Badie B. Neuro Oncol. 2022 Nov 17:noac250. doi: 10.1093/neuonc/noac250. PubMed
Elevated FSP1 protects KRAS-mutated cells from ferroptosis during tumor initiation. Muller F, Lim JKM, Bebber CM, Seidel E, Tishina S, Dahlhaus A, Stroh J, Beck J, Yapici FI, Nakayama K, Torres Fernandez L, Bragelmann J, Leprivier G, von Karstedt S. Cell Death Differ. 2023 Feb;30(2):442-456. doi: 10.1038/s41418-022-01096-8. Epub 2022 Nov 29. PubMed
BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network. Sun X, Klingbeil O, Lu B, Wu C, Ballon C, Ouyang M, Wu XS, Jin Y, Hwangbo Y, Huang YH, Somerville TDD, Chang K, Park J, Chung T, Lyons SK, Shi J, Vogel H, Schulder M, Vakoc CR, Mills AA. Nature. 2023 Jan;613(7942):195-202. doi: 10.1038/s41586-022-05551-x. Epub 2022 Dec 21. PubMed
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Associated Plasmids
HER2-driven breast cancer suppression by the JNK signaling pathway. Itah Z, Chaudhry S, Raju Ponny S, Aydemir O, Lee A, Cavanagh-Kyros J, Tournier C, Muller WJ, Davis RJ. Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2218373120. doi: 10.1073/pnas.2218373120. Epub 2023 Jan 19. PubMed
EVL and MIM/MTSS1 regulate actin cytoskeletal remodeling to promote dendritic filopodia in neurons. Parker SS, Ly KT, Grant AD, Sweetland J, Wang AM, Parker JD, Roman MR, Saboda K, Roe DJ, Padi M, Wolgemuth CW, Langlais P, Mouneimne G. J Cell Biol. 2023 May 1;222(5):e202106081. doi: 10.1083/jcb.202106081. Epub 2023 Feb 24. PubMed
Translational landscape in human early neural fate determination. Yan C, Meng Y, Yang J, Chen J, Jiang W. Development. 2023 Mar 15;150(6):dev201177. doi: 10.1242/dev.201177. Epub 2023 Mar 15. PubMed
Guide-specific loss of efficiency and off-target reduction with Cas9 variants. Zhang L, He W, Fu R, Xu H. bioRxiv. 2023 Mar 16:2023.03.16.532856. doi: 10.1101/2023.03.16.532856. Preprint. PubMed
N-terminal acetylation can stabilize proteins independent of their ubiquitination. van de Kooij B, de Vries E, Rooswinkel RW, Janssen GMC, Kok FK, van Veelen PA, Borst J. Sci Rep. 2023 Apr 1;13(1):5333. doi: 10.1038/s41598-023-32380-3. PubMed
MEK inhibition reduced vascular tumor growth and coagulopathy in a mouse model with hyperactive GNAQ. Schrenk S, Bischoff LJ, Goines J, Cai Y, Vemaraju S, Odaka Y, Good SR, Palumbo JS, Szabo S, Reynaud D, Van Raamsdonk CD, Lang RA, Boscolo E. Nat Commun. 2023 Apr 6;14(1):1929. doi: 10.1038/s41467-023-37516-7. PubMed
Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies. Guo L, Mao Q, He J, Liu X, Piao X, Luo L, Hao X, Yu H, Song Q, Xiao B, Fan D, Gao Z, Jia Y. Cell Res. 2023 Jul;33(7):497-515. doi: 10.1038/s41422-023-00798-z. Epub 2023 May 4. PubMed
The c-Src/LIST Positive Feedback Loop Sustains Tumor Progression and Chemoresistance. Wang X, Wang B, Li F, Li X, Guo T, Gao Y, Wang D, Huang W. Adv Sci (Weinh). 2023 Jul;10(20):e2300115. doi: 10.1002/advs.202300115. Epub 2023 May 8. PubMed
PRAME induces genomic instability in uveal melanoma. Harbour JW, Kurtenbach S, Sanchez M, Kuznetsoff J, Rodriguez D, Weich N, Dollar J, Cruz A, Kurtenbach S, Field M, Decatur C, Sorouri M, Lai F, Shiekhattar R, Pelaez D, Correa Z, Verdun R. Res Sq. 2023 Apr 26:rs.3.rs-2861359. doi: 10.21203/rs.3.rs-2861359/v1. Preprint. PubMed
The lipase cofactor CGI58 controls placental lipolysis. Guerrero-Santoro J, Morizane M, Oh SY, Mishima T, Goff JP, Bildirici I, Sadovsky E, Ouyang Y, Tyurin VA, Tyurina YY, Kagan VE, Sadovsky Y. JCI Insight. 2023 May 22;8(10):e168717. doi: 10.1172/jci.insight.168717. PubMed
ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion. Menolfi D, Lee BJ, Zhang H, Jiang W, Bowen NE, Wang Y, Zhao J, Holmes A, Gershik S, Rabadan R, Kim B, Zha S. bioRxiv. 2023 May 27:2023.05.26.542515. doi: 10.1101/2023.05.26.542515. Preprint. PubMed
A target discovery pipeline identified ILT3 as a target for immunotherapy of multiple myeloma. Di Meo F, Iyer A, Akama K, Cheng R, Yu C, Cesarano A, Kurihara N, Tenshin H, Aljoufi A, Marino S, Soni RK, Roda J, Sissons J, Vu LP, Guzman M, Huang K, Laskowski T, Broxmeyer HE, Roodman DG, Perna F. Cell Rep Med. 2023 Jul 18;4(7):101110. doi: 10.1016/j.xcrm.2023.101110. PubMed
Matriptase-dependent epidermal pre-neoplasm in zebrafish embryos caused by a combination of hypotonic stress and epithelial polarity defects. Hatzold J, Nett V, Brantsch S, Zhang JL, Armistead J, Wessendorf H, Stephens R, Humbert PO, Iden S, Hammerschmidt M. PLoS Genet. 2023 Aug 11;19(8):e1010873. doi: 10.1371/journal.pgen.1010873. eCollection 2023 Aug. PubMed
CRISPR-Cas9 Library Screening Identifies Novel Molecular Vulnerabilities in KMT2A-Rearranged Acute Lymphoblastic Leukemia. Schneider P, Wander P, Arentsen-Peters STCJM, Vrenken KS, Rockx-Brouwer D, Adriaanse FRS, Hoeve V, Paassen I, Drost J, Pieters R, Stam RW. Int J Mol Sci. 2023 Aug 25;24(17):13207. doi: 10.3390/ijms241713207. PubMed
Protocol to capture transcription factor-mediated 3D chromatin interactions using affinity tag-based BL-HiChIP. Ren R, Hua Y, Wang H. STAR Protoc. 2023 Dec 15;4(4):102589. doi: 10.1016/j.xpro.2023.102589. Epub 2023 Sep 21. PubMed
The cancer testes antigen, HORMAD1, limits genomic instability in cancer cells by protecting stalled replication forks. Herrera LR, Johnson RA, McGlynn K, Gibbs ZA, Davis AJ, Whitehurst AW. J Biol Chem. 2023 Nov;299(11):105348. doi: 10.1016/j.jbc.2023.105348. Epub 2023 Oct 12. PubMed
Active growth signaling promotes senescence and cancer cell sensitivity to CDK7 inhibition. Wilson GA, Vuina K, Sava G, Huard C, Meneguello L, Coulombe-Huntington J, Bertomeu T, Maizels RJ, Lauring J, Kriston-Vizi J, Tyers M, Ali S, Bertoli C, de Bruin RAM. Mol Cell. 2023 Nov 16;83(22):4078-4092.e6. doi: 10.1016/j.molcel.2023.10.017. Epub 2023 Nov 16. PubMed
Unraveling the Mechanisms of Sensitivity to Anti-FGF Therapies in Imatinib-Resistant Gastrointestinal Stromal Tumors (GIST) Lacking Secondary KIT Mutations. Boichuk S, Dunaev P, Skripova V, Galembikova A, Bikinieva F, Shagimardanova E, Gazizova G, Deviatiiarov R, Valeeva E, Mikheeva E, Vasilyeva M, Kopnin P, Strelnikov V, Kiyamova R. Cancers (Basel). 2023 Nov 9;15(22):5354. doi: 10.3390/cancers15225354. PubMed
Transcriptional inhibition after irradiation occurs preferentially at highly expressed genes in a manner dependent on cell cycle progression. Chen Z, Wang X, Gao X, Arslanovic N, Chen K, Tyler J. bioRxiv [Preprint]. 2023 Nov 20:2023.11.20.567799. doi: 10.1101/2023.11.20.567799. PubMed
Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis. Yang X, Gu C, Cai J, Li F, He X, Luo L, Xiao W, Hu B, Hu J, Qian H, Ren S, Zhang L, Zhu X, Yang L, Yang J, Yang Z, Zheng Y, Huang X, Wang Z. Redox Biol. 2024 Feb;69:103002. doi: 10.1016/j.redox.2023.103002. Epub 2023 Dec 17. PubMed
Transcriptional determinants of lipid mobilization in human adipocytes. Ludzki AC, Hansen M, Zareifi D, Jalkanen J, Huang Z, Omar-Hmeadi M, Renzi G, Klingelhuber F, Boland S, Ambaw YA, Wang N, Damdimopoulos A, Liu J, Jernberg T, Petrus P, Arner P, Krahmer N, Fan R, Treuter E, Gao H, Ryden M, Mejhert N. Sci Adv. 2024 Jan 5;10(1):eadi2689. doi: 10.1126/sciadv.adi2689. Epub 2024 Jan 3. PubMed
Heat shock factor 1 directly regulates transsulfuration pathway to promote prostate cancer proliferation and survival. Hauck JS, Moon D, Jiang X, Wang ME, Zhao Y, Xu L, Quang H, Butler W, Chen M, Macias E, Gao X, He Y, Huang J. Commun Biol. 2024 Jan 3;7(1):9. doi: 10.1038/s42003-023-05727-9. PubMed
Mesothelin Secretion by Pancreatic Cancer Cells Co-opts Macrophages and Promotes Metastasis. Luckett T, Abudula M, Ireland L, Glenn M, Bellomo G, Stafferton R, Halloran C, Ghaneh P, Jones R, Schmid MC, Mielgo A. Cancer Res. 2024 Feb 15;84(4):527-544. doi: 10.1158/0008-5472.CAN-23-1542. PubMed
Mitochondria-Targeted Oligomeric alpha-Synuclein Induces TOM40 Degradation and Mitochondrial Dysfunction in Parkinson's Disease and Parkinsonism-Dementia of Guam. Hegde M, Vasquez V, Kodavati M, Mitra J, Vendula I, Hamilton D, Garruto R, Rao KS. Res Sq [Preprint]. 2024 Feb 21:rs.3.rs-3970470. doi: 10.21203/rs.3.rs-3970470/v1. PubMed
DeltaNp63 regulates MDSC survival and metabolism in triple-negative breast cancer. Kim U, Debnath R, Maiz JE, Rico J, Sinha S, Blanco MA, Chakrabarti R. iScience. 2024 Feb 29;27(4):109366. doi: 10.1016/j.isci.2024.109366. eCollection 2024 Apr 19. PubMed
A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module controls centrosome function. Ganga AK, Sweeney LK, Ramos AR, Bishop CS, Hamel V, Guichard P, Breslow DK. bioRxiv [Preprint]. 2024 Apr 3:2024.04.02.587836. doi: 10.1101/2024.04.02.587836. PubMed
RNA/DNA Binding Protein TDP43 Regulates DNA Mismatch Repair Genes with Implications for Genome Stability. Provasek VE, Bacolla A, Rangaswamy S, Mitra J, Kodavati M, Yusuf IO, Malojirao VH, Vasquez V, Britz GW, Li GM, Xu Z, Mitra S, Garruto RM, Tainer JA, Hegde ML. bioRxiv [Preprint]. 2024 May 17:2024.05.16.594552. doi: 10.1101/2024.05.16.594552. PubMed

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