pHIS3p:mEos2-Tub1+3'UTR::TRP1
(Plasmid
#50652)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50652 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemEos2-Tub1
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Alt namemEos2-a-tubulin
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2144
- Promoter HIS3p
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Tag
/ Fusion Protein
- mEos2 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHIS3p:mEos2-Tub1+3'UTR::TRP1 was a gift from Wei-Lih Lee (Addgene plasmid # 50652 ; http://n2t.net/addgene:50652 ; RRID:Addgene_50652) -
For your References section:
Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae. Markus SM, Omer S, Baranowski K, Lee WL. Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 Apr 28. 10.1111/tra.12276 PubMed 25711127