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pHIS3p:mRuby2-Tub1+3'UTR::LEU2
(Plasmid #50645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRuby2-Tub1
  • Alt name
    mRuby2-a-tubulin
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2177
  • Promoter HIS3p
  • Tag / Fusion Protein
    • mRuby2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIS3p:mRuby2-Tub1+3'UTR::LEU2 was a gift from Wei-Lih Lee (Addgene plasmid # 50645 ; http://n2t.net/addgene:50645 ; RRID:Addgene_50645)

  • For your References section:

    Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae. Markus SM, Omer S, Baranowski K, Lee WL. Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 Apr 28. 10.1111/tra.12276 PubMed 25711127
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