pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
(Plasmid
#50626)
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Purpose(Empty Backbone) pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50626 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
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Modifications to backbonecloned the N terminal sequence for the RipTAL (based on information from doi: 10.1111/nph.12324) in place of the TAL N term between the SbfI and XhoI sites
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Vector typeMammalian Expression, Lentiviral, TALEN
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers was a gift from Andrew Scharenberg (Addgene plasmid # 50626 ; http://n2t.net/addgene:50626 ; RRID:Addgene_50626)
