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Addgene

pNS10
(Plasmid #50618)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTC14
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 3007
  • Total vector size (bp) 3101
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    10mg/L Tet
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NS10
  • Species
    Xanthamonas
  • Insert Size (bp)
    94

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer caagcctgattgggagaaaa
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNS10 was a gift from Daniel Voytas (Addgene plasmid # 50618 ; http://n2t.net/addgene:50618 ; RRID:Addgene_50618)
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