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Addgene

pY3
(Plasmid #50606)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRBS-01
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    tyrB
  • Insert Size (bp)
    1193
  • Promoter lacUV5

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer TGTGGAATTGTGAGCGGATA
  • 3′ sequencing primer TAATGCGGTCAATTCAGCAA
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tyrA
  • Insert Size (bp)
    1121
  • Promoter lacUV5

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer TGTGGAATTGTGAGCGGATA
  • 3′ sequencing primer ATCGACGATGCAGCCGAG
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    aroC
  • Insert Size (bp)
    1085
  • Promoter lacUV5

Cloning Information for Gene/Insert 3

  • Cloning method Unknown
  • 5′ sequencing primer TGTGGAATTGTGAGCGGATA
  • 3′ sequencing primer CCTGACGCCAGAAGCATT
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    aroA
  • Insert Size (bp)
    1283
  • Promoter lacUV5

Cloning Information for Gene/Insert 4

  • Cloning method Unknown
  • 5′ sequencing primer TGTGGAATTGTGAGCGGATA
  • 3′ sequencing primer GTTAAGCGAATCGGCAAGG
    • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    aroL
  • Insert Size (bp)
    524
  • Promoter lacUV5

Cloning Information for Gene/Insert 5

  • Cloning method Unknown
  • 5′ sequencing primer TGTGGAATTGTGAGCGGATA
  • 3′ sequencing primer TTTGAGCGTCAGATTTCGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pY3 was a gift from Jay Keasling (Addgene plasmid # 50606 ; http://n2t.net/addgene:50606 ; RRID:Addgene_50606)

  • For your References section:

    Modular engineering of L-tyrosine production in Escherichia coli. Juminaga D, Baidoo EE, Redding-Johanson AM, Batth TS, Burd H, Mukhopadhyay A, Petzold CJ, Keasling JD. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21. 10.1128/AEM.06017-11 PubMed 22020510
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