pCBC-MT2T3
(Plasmid #50594)

• Purpose: CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
• Depositing Lab: Qi-Jun Chen
• Publication: Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Backbone

• Vector backbone: pGWC
• Vector type: Plant Expression, CRISPR ; PCR template

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T2, gRNA scaffold, TaU3t plus, U6-26p, T3
• Species: Synthetic

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry