pDBD-M
(Plasmid #50572)

• Purpose: Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571
• Depositing Lab: Alexander Varshavsky
• Publication: Turner et al Science. 2000 Sep 22;289(5487):2117-20.

Backbone

• Vector backbone: pRS426 Gal1
• Backbone size w/o insert (bp): 6400
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
• Species: Synthetic; E. Coli
• Insert Size (bp): 5200
• Promoter: Gal1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a H to T mutation in front of the MetBetaGal. The mutation should not affect function.