pDBD-R
(Plasmid
#50571)
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PurposeDouble Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50571 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS426 Gal1
- Backbone size w/o insert (bp) 6400
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
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SpeciesSynthetic; E. Coli
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Insert Size (bp)5200
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MutationM1R (B-gal)
- Promoter Gal1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDBD-R was a gift from Alexander Varshavsky (Addgene plasmid # 50571 ; http://n2t.net/addgene:50571 ; RRID:Addgene_50571) -
For your References section:
Detecting and measuring cotranslational protein degradation in vivo. Turner GC, Varshavsky A. Science. 2000 Sep 22;289(5487):2117-20. 10.1126/science.289.5487.2117 PubMed 11000112