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PurposeRetroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMy
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Modifications to backboneAdded coding regions of mouse CTCF followed by T2A and mOrange
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameAmpicilin
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Insert Size (bp)790
Gene/Insert 2
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Gene/Insert nameMouse CTCF Exons
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Insert Size (bp)2208
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMy-MouseCTCFbiotag-T2A-mOrange was a gift from Rafael Casellas (Addgene plasmid # 50564 ; http://n2t.net/addgene:50564 ; RRID:Addgene_50564) -
For your References section:
A genome-wide map of CTCF multivalency redefines the CTCF code. Nakahashi H, Kwon KR, Resch W, Vian L, Dose M, Stavreva D, Hakim O, Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair RD, Pugh BF, Lobanenkov V, Hager GL, Casellas R. Cell Rep. 2013 May 30;3(5):1678-89. doi: 10.1016/j.celrep.2013.04.024. Epub 2013 May 23. 10.1016/j.celrep.2013.04.024 PubMed 23707059