pIZ-Flag6His-Siwi
(Plasmid #50560)

• Purpose: Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells
• Depositing Lab: Yukihide Tomari
• Publication: Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.1261/rna.1452209. Epub 2009 May 21.

Backbone

• Vector backbone: pIZ/V5-His
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 5578
• Vector type: Insect Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Siwi
• Alt name: Aubergine
• Entrez Gene: Aubergine (a.k.a. Piwi, SIWI, siwi1)
• Promoter: OpIE2
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • 6xHIS (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EBV-rev
• 3′ sequencing primer: V5-Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene origin is from Susumu Katsuma at The Univ. of Tokyo.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry