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pIZ-Flag6His-BmAgo3
(Plasmid #50559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIZ/V5-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 5659
  • Vector type
    Insect Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BmAgo3
  • Species
    Bombyx mori
  • GenBank ID
  • Promoter OpIE2
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • 6xHIS (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene origin is from Susumu Katsuma at The Univ. of Tokyo.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIZ-Flag6His-BmAgo3 was a gift from Yukihide Tomari (Addgene plasmid # 50559 ; http://n2t.net/addgene:50559 ; RRID:Addgene_50559)

  • For your References section:

    The Bombyx ovary-derived cell line endogenously expresses PIWI/PIWI-interacting RNA complexes. Kawaoka S, Hayashi N, Suzuki Y, Abe H, Sugano S, Tomari Y, Shimada T, Katsuma S. RNA. 2009 Jul;15(7):1258-64. doi: 10.1261/rna.1452209. Epub 2009 May 21. 10.1261/rna.1452209 PubMed 19460866
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