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Addgene

pCAGEN-SBP-DICER1
(Plasmid #50558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGEN
  • Backbone manufacturer
    Connie Cepko, Addgene plasmid 11160
  • Backbone size w/o insert (bp) 4798
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dicer1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Dicer1 (a.k.a. 1110006F08Rik, D12Ertd7e, aviD, mKIAA0928)
  • Promoter CAG
  • Tag / Fusion Protein
    • SBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene origin is from Kumiko Ui-tei at The Univ. of Tokyo
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In-Fusion® HD Cloning from Clontech used for inserting Dicer1-SBP into EcoRI cut pCAGEN.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGEN-SBP-DICER1 was a gift from Yukihide Tomari (Addgene plasmid # 50558 ; http://n2t.net/addgene:50558 ; RRID:Addgene_50558)

  • For your References section:

    Dicer is dispensable for asymmetric RISC loading in mammals. Betancur JG, Tomari Y. RNA. 2012 Jan;18(1):24-30. doi: 10.1261/rna.029785.111. Epub 2011 Nov 21. 10.1261/rna.029785.111 PubMed 22106413
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