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pAFW-Ago2
(Plasmid #50554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAFW
  • Backbone manufacturer
    The Drosophila Gateway™ Vector Collection
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Argonaute2
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    AGO2 (a.k.a. Dmel_CG7439, AG02, AGO 2, AGO-2, AGO1, Ago-2, Ago2, Ago2a, CG13452, CG7439, Dm Ago2, DmAgo2, Dmel\CG7439, ago, ago-2, ago2, argonaute2, dAGO2, dAgo2, eIF2C2)
  • Promoter Actin5C
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene origin is from Phillip Zamore at Univ. of Massachusetts.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that when compared to GenBank reference sequence NP_648775.1, the Ago2 sequence in this plasmid is missing amino acids 49-54 (LQQPQQ).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAFW-Ago2 was a gift from Yukihide Tomari (Addgene plasmid # 50554 ; http://n2t.net/addgene:50554 ; RRID:Addgene_50554)

  • For your References section:

    Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. Iwasaki S, Kobayashi M, Yoda M, Sakaguchi Y, Katsuma S, Suzuki T, Tomari Y. Mol Cell. 2010 Jul 30;39(2):292-9. doi: 10.1016/j.molcel.2010.05.015. Epub 2010 Jun 3. 10.1016/j.molcel.2010.05.015 PubMed 20605501
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