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pAFW-Ago1
(Plasmid #50553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAFW
  • Backbone manufacturer
    The Drosophila Gateway™ Vector Collection
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Argonaute1
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    AGO1 (a.k.a. Dmel_CG6671, AGO-1, Ago, Ago-1, Ago1, Agp1, CG6671, Dm Ago1, DmAgo1, Dmel\CG6671, MRE20, ago-1, ago1, ago1-1, anon-WO0257455.29, argonaute1, dAGO1, dAgo1, dmAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121)
  • Promoter Actin5C
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene origin is from Phillip Zamore at Univ. of Massachusetts.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The closest reference sequence for the Ago1 insert in this plasmid appears to be NM_079010.4, Argonaute-1, isoform B.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAFW-Ago1 was a gift from Yukihide Tomari (Addgene plasmid # 50553 ; http://n2t.net/addgene:50553 ; RRID:Addgene_50553)

  • For your References section:

    Structural determinants of miRNAs for RISC loading and slicer-independent unwinding. Kawamata T, Seitz H, Tomari Y. Nat Struct Mol Biol. 2009 Sep;16(9):953-60. doi: 10.1038/nsmb.1630. Epub 2009 Aug 16. 10.1038/nsmb.1630 PubMed 19684602
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