pJT119b
(Plasmid #50551)

• Purpose: Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter
• Depositing Lab: Jeffrey Tabor
• Publication: Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.

Backbone

• Vector backbone: pProTet.E333
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 1900
• Total vector size (bp): 6443
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CcaS
• Species: Synechocystis sp. 6803
• Insert Size (bp): 2262

Gene/Insert 2

• Gene/Insert name: CcaR
• Species: Synechocystis sp. 6803
• Insert Size (bp): 705

Gene/Insert 3

• Gene/Insert name: sfGFP
• Alt name: Superfolder GFP
• Species: Synthetic
• Insert Size (bp): 720

Resource Information

• Supplemental Documents:
  • Annotated plasmid sequence (genbank format)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry