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PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50551 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepProTet.E333
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 1900
- Total vector size (bp) 6443
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCcaS
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SpeciesSynechocystis sp. 6803
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Insert Size (bp)2262
Gene/Insert 2
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Gene/Insert nameCcaR
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SpeciesSynechocystis sp. 6803
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Insert Size (bp)705
Gene/Insert 3
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Gene/Insert namesfGFP
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Alt nameSuperfolder GFP
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SpeciesSynthetic
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Insert Size (bp)720
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJT119b was a gift from Jeffrey Tabor (Addgene plasmid # 50551 ; http://n2t.net/addgene:50551 ; RRID:Addgene_50551) -
For your References section:
Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals. Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ. Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884. 10.1038/nmeth.2884 PubMed 24608181