pBL6
(Plasmid
#50549)
-
PurposeExpresses mCherry under the PLtetO-1 promoter
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 50549 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSB4A3
-
Backbone manufacturerRegistry of Standard Biological Parts
- Backbone size w/o insert (bp) 3296
- Total vector size (bp) 4242
-
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namemCherry
-
SpeciesSynthetic
-
Insert Size (bp)946
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: Addgene's quality control sequencing found a few mismatches compared to the depositor's full sequence, but they should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBL6 was a gift from Jeffrey Tabor (Addgene plasmid # 50549 ; http://n2t.net/addgene:50549 ; RRID:Addgene_50549) -
For your References section:
Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals. Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ. Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884. 10.1038/nmeth.2884 PubMed 24608181