pDEST40-MFRN2
(Plasmid #50546)

• Purpose: Expression of MFRN2 gene in mammalian cells
• Depositing Lab: Vamsi Mootha
• Publication: Baughman et al Nature. 2011 Jun 19. ():.

Backbone

• Vector backbone: pcDNA/DEST40/C-V5
• Backbone manufacturer: Life technologies
• Backbone size w/o insert (bp): 7143
• Total vector size (bp): 8367
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Mrfn2
• Alt name: SLC25A28
• Species: H. sapiens (human)
• Insert Size (bp): 1224
• Entrez Gene: SLC25A28 (a.k.a. MFRN2, MRS3/4, MRS4L, NPD016)
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F, T7
• 3′ sequencing primer: V5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry