Skip to main content
Addgene

pIL2R FAK Y397F
(Plasmid #50525)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50525 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMVIL2R(pIL2R)
  • Backbone manufacturer
    Bruce Howard Lab, PubMed ID 1373146
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    protein tyrosine kinase, mutated
  • Alt name
    FAK
  • Alt name
    PTK2
  • Species
    M. musculus (mouse)
  • Mutation
    Y397F
  • Promoter CMV
  • Tag / Fusion Protein
    • IL-2R (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (not destroyed)
  • 3′ cloning site unknown (not destroyed)
  • 5′ sequencing primer IL2R FW (TCCTGCCTCGTCACAACAAC)
  • 3′ sequencing primer Il2R AS2 (CCTTAGAGCTTTAAATCTCTG)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector contains truncated IL-2R alpha subunit without cytoplasmic domain (Tac antigen) for membrane localization and clustering

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIL2R FAK Y397F was a gift from Kenneth Yamada (Addgene plasmid # 50525 ; http://n2t.net/addgene:50525 ; RRID:Addgene_50525)

  • For your References section:

    PTEN interactions with focal adhesion kinase and suppression of the extracellular matrix-dependent phosphatidylinositol 3-kinase/Akt cell survival pathway. Tamura M, Gu J, Danen EH, Takino T, Miyamoto S, Yamada KM. J Biol Chem. 1999 Jul 16;274(29):20693-703. 10.1074/jbc.274.29.20693 PubMed 10400703
Related items:
Commonly requested with: