pGFP FAK
(Plasmid #50515)

• Purpose: This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
• Depositing Lab: Kenneth Yamada
• Publication: Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Backbone

• Vector backbone: pGZ21dxZ
• Backbone manufacturer: Yamada Lab, Tamura et al., Science 280: 1614-7 (1998)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: protein tyrosine kinase
• Alt name: FAK
• Alt name: PTK2
• Species: M. musculus (mouse)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: GFP FW (AAAGACCCCAACGAGAAGCG)
• 3′ sequencing primer: GFP ASO (TTGTAACCATTATAAGCTGC)

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Fluorophore encoded by this plasmid is ABB59962.1 with G66A.