f.pRST1
(Plasmid #50494)

• Purpose: Modified pBluescript containing a telomeric insert.
• Depositing Lab: Jack Griffith
• Publication: Arat et al J Biol Chem. 2012 Dec 7;287(50):41583-94. doi: 10.1074/jbc.M112.415984. Epub 2012 Oct 20.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Agilent
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 3091
• Modifications to backbone: See putative plasmid sequence.
• Vector type: Structural DNA substrate/material

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Growth instructions: Telomeric inserts are unstable during prolonged culture. Recommend maintaining plasmid as a DNA stock and growing from a fresh transformation with minimal incubation times.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TTAGGG(n) Track
• Insert Size (bp): 136
• Promoter: T3 and T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (not destroyed)
• 3′ cloning site: BsmBI (not destroyed)
• 5′ sequencing primer: AAT TAA CCC TCA CTA AAG GG
• 3′ sequencing primer: TAA TAC GAC TCA CTA TAG GG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry