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Addgene

pEBG MKK3b (Glu)
(Plasmid #50451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50451 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBG
  • Backbone manufacturer
    David Baltimore (Addgene Plasmid # 22227)
  • Backbone size w/o insert (bp) 6034
  • Total vector size (bp) 7075
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MKK3b
  • Alt name
    MAP2K3 mitogen-activated protein kinase kinase 3
  • Alt name
    MKK3 isoform B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1041
  • Mutation
    changed Serine 218 to Glutamic Acid, changed Threonine 222 to Glutamic Acid
  • GenBank ID
    NM_145109.2
  • Entrez Gene
    MAP2K3 (a.k.a. MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2)
  • Promoter SV40
  • Tag / Fusion Protein
    • Gst (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBG MKK3b (Glu) was a gift from Roger Davis (Addgene plasmid # 50451 ; http://n2t.net/addgene:50451 ; RRID:Addgene_50451)

  • For your References section:

    Molecular determinants that mediate selective activation of p38 MAP kinase isoforms. Enslen H, Brancho DM, Davis RJ. EMBO J. 2000 Mar 15. 19(6):1301-11. 10.1093/emboj/19.6.1301 PubMed 10716930
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