pCDNA3 Flag MKK3b(Glu, delete LXL)
(Plasmid
#50450)
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Purposeactivates p38 in the signaling pathway
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50450 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6441
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMKK3b
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Alt nameMAP2K3 mitogen-activated protein kinase kinase 3
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Alt nameMKK3 isoform B
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SpeciesH. sapiens (human)
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Insert Size (bp)1041
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Mutationdeleted L25-I27, changed Serine 218 to Glutamic Acid, changed Threonine 222 to Glutamic Acid
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GenBank IDNM_145109.2
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Entrez GeneMAP2K3 (a.k.a. MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3 Flag MKK3b(Glu, delete LXL) was a gift from Roger Davis (Addgene plasmid # 50450 ; http://n2t.net/addgene:50450 ; RRID:Addgene_50450) -
For your References section:
Molecular determinants that mediate selective activation of p38 MAP kinase isoforms. Enslen H, Brancho DM, Davis RJ. EMBO J. 2000 Mar 15. 19(6):1301-11. 10.1093/emboj/19.6.1301 PubMed 10716930