pCDNA3 Flag MKK3b(Glu)
(Plasmid #50449)

• Purpose: activates p38 in the signaling pathway
• Depositing Lab: Roger Davis
• Publication: Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6441
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MKK3b
• Alt name: MAP2K3 mitogen-activated protein kinase kinase 3
• Alt name: MKK3 isoform B
• Species: H. sapiens (human)
• Insert Size (bp): 1041
• Mutation: changed Serine 218 to Glutamic Acid, changed Threonine 222 to Glutamic Acid
• GenBank ID: NM_145109.2
• Entrez Gene: MAP2K3 (a.k.a. MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry