pET28a-Ec.coaBC (pCLK1210)
(Plasmid #50448)

• Purpose: Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase
• Depositing Labs: Tadhg Begley, Erick Strauss
• Publication: Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6. Epub 2001 Mar 13.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPCS PPCDC
• Alt name: phosphopantothenoylcysteine synthetase phosphopantothenoylcysteine decarboxylase
• Species: E coli
• Promoter: T7
• Tag / Fusion Protein:
  • 6XHIS tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry