pLenti4/TO-hEPO
(Plasmid #50437)

• Purpose: express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system
• Depositing Lab: Juan Melero-Martin
• Publication: Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi: 10.1182/blood-2011-08-372946. Epub 2011 Sep 21.

Backbone

• Vector backbone: pLenti4/TO/V5-DEST gateway vector
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 8598
• Total vector size (bp): 9200
• Vector type: Mammalian Expression, Lentiviral ; Tetracycline-regulatable (T-REx)
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EPO
• Alt name: erythropoietin
• Alt name: MVCD2
• Species: H. sapiens (human)
• Insert Size (bp): 582
• Entrez Gene: EPO (a.k.a. DBAL, ECYT5, EP, MVCD2)
• Promoter: CMV/TO

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human erythropoietin (hEPO) ORF with stop codon (582 bp; Ultimate ORF clone number IOH7334) from GeneCopoeia ORF cDNA clones in pDONR vector (GeneCopoeia; GC-A1011)
Because the stop codon is inserted before V5 epitope, the V5 tag on the pLenti6.3/V5-DEST vector won’t be transcribed.