pCVL SFFV Sbf-Sal linker
(Plasmid
#50434)
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Purpose(Empty Backbone) Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50434 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCVL TALEN(Nd154C63) SFFV
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Modifications to backboneClones out TALEN cloning region between SbfI and SalI sites and replaced with a short linker
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Vector typeMammalian Expression, Lentiviral
- Promoter SFFV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVL SFFV Sbf-Sal linker was a gift from Andrew Scharenberg (Addgene plasmid # 50434 ; http://n2t.net/addgene:50434 ; RRID:Addgene_50434) -
For your References section:
megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering. Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM. Nucleic Acids Res. 2013 Nov 26. 10.1093/nar/gkt1224 PubMed 24285304
