pTALEN(Nd154C63) SFFV
(Plasmid
#50431)
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Purpose(Empty Backbone) pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCVL UCOE.SFFV.BFP.2a
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Modifications to backboneModified insert from the Golden gate cloning system pTAL3 vector to clone truncated version of TAL (Nd154C63) with Fok into vector downstream of UCOE.SFFV.BFP.2a, along with an HA tag and NLS
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Vector typeMammalian Expression, Lentiviral, TALEN
- Promoter SFFV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTALEN(Nd154C63) SFFV was a gift from Andrew Scharenberg (Addgene plasmid # 50431 ; http://n2t.net/addgene:50431 ; RRID:Addgene_50431)
