pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
(Plasmid
#50421)
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Purpose(Empty Backbone) pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTAL(Nd154C63) SFFV Xba-Sal linker
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Modifications to backboneCloned an Age site into the N-terminal TALE region and replaced the lacZ/TAL RVD cloning region with a short Age-Xho linker for easy cloning of the full TALE RVD sequence.
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Vector typeMammalian Expression, Lentiviral, TALEN
- Promoter SFFV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers was a gift from Andrew Scharenberg (Addgene plasmid # 50421 ; http://n2t.net/addgene:50421 ; RRID:Addgene_50421) -
For your References section:
megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering. Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM. Nucleic Acids Res. 2013 Nov 26. 10.1093/nar/gkt1224 PubMed 24285304
