pExodus iRFP-T2A-Trex2
(Plasmid #50419)

• Purpose: Coexpresses Trex2 and iRFP in mammalian cells.
• Depositing Lab: Andrew Scharenberg
• Publication: Trex2 plasmids (unpublished)

Backbone

• Vector backbone: pExodus CMV.Trex2
• Modifications to backbone: Cloned iRFP-T2A upstream of Trex2 using the NheI site and adding a new AgeI site.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: iRFP
• Insert Size (bp): 948
• Promoter: CMV
• Tag / Fusion Protein:
  • T2A (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Gene/Insert 2

• Gene/Insert name: Trex2
• Tag / Fusion Protein:
  • T2A (N terminal on insert)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry