SeeSaw Reporter (SSR1.0)
(Plasmid
#50392)
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PurposeSSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50392 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 6417
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Modifications to backboneInclude WPRE enhancer after SSR1.0 insert.
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Vector typeMammalian Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSSR1.0
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SpeciesSynthetic
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Insert Size (bp)2500
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SeeSaw Reporter (SSR1.0) was a gift from Pablo Huertas Sánchez (Addgene plasmid # 50392 ; http://n2t.net/addgene:50392 ; RRID:Addgene_50392) -
For your References section:
New tools to study DNA double-strand break repair pathway choice. Gomez-Cabello D, Jimeno S, Fernandez-Avila MJ, Huertas P. PLoS One. 2013 Oct 14;8(10):e77206. doi: 10.1371/journal.pone.0077206. 10.1371/journal.pone.0077206 PubMed 24155929
Map uploaded by the depositor.
