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Addgene

pET28a-Ec.coaD (pESC106)
(Plasmid #50388)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50388 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAT
  • Alt name
    phosphopantetheine adenylyltransferase
  • Species
    E coli
  • GenBank ID
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-Ec.coaD (pESC106) was a gift from Tadhg Begley & Erick Strauss (Addgene plasmid # 50388 ; http://n2t.net/addgene:50388 ; RRID:Addgene_50388)

  • For your References section:

    The antibiotic activity of N-pentylpantothenamide results from its conversion to ethyldethia-coenzyme a, a coenzyme a antimetabolite. Strauss E, Begley TP. J Biol Chem. 2002 Dec 13;277(50):48205-9. Epub 2002 Oct 7. 10.1074/jbc.M204560200 PubMed 12372838
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