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PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePanK
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Alt namepantothenate kinase
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SpeciesE coli
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GenBank IDAAC76952.1
- Promoter T7
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Tag
/ Fusion Protein
- 6XHis-tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-Ec.coaA was a gift from Erick Strauss (Addgene plasmid # 50386 ; http://n2t.net/addgene:50386 ; RRID:Addgene_50386) -
For your References section:
Characterization of a new pantothenate kinase isoform from Helicobacter pylori. Brand LA, Strauss E. J Biol Chem. 2005 May 27;280(21):20185-8. Epub 2005 Mar 28. 10.1074/jbc.C500044200 PubMed 15795230
Map uploaded by the depositor.
