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hCEP120-Myc
(Plasmid #50381)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50381 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO/myc-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5151
  • Total vector size (bp) 8095
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human CEP120
  • Alt name
    CCDC100
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2964
  • Mutation
    This hCEP120 contains 4 SNPs (rs6876883, rs6595440, rs1047437, rs1047438) in our clone comparing to NM_153223.
  • GenBank ID
    NM_153223
  • Entrez Gene
    CEP120 (a.k.a. CCDC100)
  • Promoter CMV-2xTetO
  • Tag / Fusion Protein
    • Myc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are 4 different nucleotides in our clone comparing to NM_153223. These nucleotides could all be found in dbSNP (rs6876883, rs6595440, rs1047437, rs1047438). Sequence of the entire ORF is available for download.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCEP120-Myc was a gift from Tang Tang (Addgene plasmid # 50381 ; http://n2t.net/addgene:50381 ; RRID:Addgene_50381)

  • For your References section:

    CEP120 interacts with CPAP and positively regulates centriole elongation. Lin YN, Wu CT, Lin YC, Hsu WB, Tang CJ, Chang CW, Tang TK. J Cell Biol. 2013 Jul 22;202(2):211-9. doi: 10.1083/jcb.201212060. Epub 2013 Jul 15. 10.1083/jcb.201212060 PubMed 23857771
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