pME-hygro-SNAP-FLAG-CD59
(Plasmid #50379)

• Purpose: Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammalian cells
• Depositing Lab: Reika Watanabe
• Publication: pME-hygro-SNAP-FLAG-CD59 (unpublished)

Backbone

• Vector backbone: pME-hygro(+)
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6400
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNAP-FLAG-tagged CD59
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• Entrez Gene: CD59 (a.k.a. 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20)
• Promoter: SR alpha
• Tags / Fusion Proteins:
  • SNAP (N terminal on backbone)
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: pcDL-F (5'-GTTGCCTTTACTTCTAGGCCT-3')
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Prof. Taroh Kinoshita, Osaka University, Japan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry