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pME-puro-Venus-FLAG-CD59
(Plasmid #50377)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME-puro-FLAG-CD59
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6200
  • Modifications to backbone
    Venus was amplified by PCR from pBS7 (Yeast Resource Center) using upper and lower primers both containing SbfI sites and integrated into the pME-puro-FLAG-CD59 plasmid at the SbfI site.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Venus
  • Alt name
    Venus-FLAG-CD59
  • Species
    Synthetic
  • Insert Size (bp)
    700
  • Promoter SR alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site SbfI (not destroyed)
  • 5′ sequencing primer agtaaaggagaagaacttttc
  • 3′ sequencing primer tttgtatagttcatccatgcc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please click View Sequences link for full plasmid sequence of vector backbone pME-puro-FLAG-CD59.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-puro-Venus-FLAG-CD59 was a gift from Reika Watanabe (Addgene plasmid # 50377 ; http://n2t.net/addgene:50377 ; RRID:Addgene_50377)

  • For your References section:

    Exit of GPI-anchored proteins from the ER differs in yeast and mammalian cells. Rivier AS, Castillon GA, Michon L, Fukasawa M, Romanova-Michaelides M, Jaensch N, Hanada K, Watanabe R. Traffic. 2010 Aug;11(8):1017-33. doi: 10.1111/j.1600-0854.2010.01081.x. Epub 2010 May 11. 10.1111/j.1600-0854.2010.01081.x PubMed 20477992
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