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Addgene

pME-puro-CLIP-FLAG-CD59
(Plasmid #50375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME-puro-FLAG-CD59
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6094
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CLIP
  • Species
    Synthetic
  • Insert Size (bp)
    546
  • Promoter SR alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site SbfI (not destroyed)
  • 5′ sequencing primer atg gac aaa gac tgc gaa atg
  • 3′ sequencing primer acc cag ccc agg ctt gcc cag
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Prof. Kai Johnsson at the Institute of Chemical Sciences and Engineering of the École Polytechnique Fédérale de Lausanne (EPFL), Switzerland

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-puro-CLIP-FLAG-CD59 was a gift from Reika Watanabe (Addgene plasmid # 50375 ; http://n2t.net/addgene:50375 ; RRID:Addgene_50375)

  • For your References section:

    Apical sorting of lysoGPI-anchored proteins occurs independent of association with detergent-resistant membranes but dependent on their N-glycosylation. Castillon GA, Michon L, Watanabe R. Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 10.1091/mbc.E13-03-0160. Epub 2013 Apr 24. 10.1091/mbc.E13-03-0160 PubMed 23615438
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