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pME-hygro-hPGAP3-3HA
(Plasmid #50373)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME-hygro(+)
  • Total vector size (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGAP3 post-GPI attachment to proteins 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • Entrez Gene
    PGAP3 (a.k.a. AGLA546, CAB2, PERLD1, PP1498, hCOS16)
  • Promoter SR alpha
  • Tag / Fusion Protein
    • 3x HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pcDL-F (5'-GTTGCCTTTACTTCTAGGCCT-3')
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Prof. Taroh Kinoshita

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-hygro-hPGAP3-3HA was a gift from Reika Watanabe (Addgene plasmid # 50373 ; http://n2t.net/addgene:50373 ; RRID:Addgene_50373)

  • For your References section:

    Apical sorting of lysoGPI-anchored proteins occurs independent of association with detergent-resistant membranes but dependent on their N-glycosylation. Castillon GA, Michon L, Watanabe R. Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 10.1091/mbc.E13-03-0160. Epub 2013 Apr 24. 10.1091/mbc.E13-03-0160 PubMed 23615438
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