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Addgene

pLex 983-Micu2
(Plasmid #50372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PLEX 983
  • Backbone size w/o insert (bp) 9379
  • Total vector size (bp) 9002
  • Modifications to backbone
    THE SAME AS PLEX 970 BUT THERE IS BLASTICIDIN INSTEAD OF PUROMYCIN GENE THERE.
  • Vector type
    Mammalian Expression ; LENTIVIRUS
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MICU2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1305
  • GenBank ID
    NM_152726
  • Entrez Gene
    MICU2 (a.k.a. 1110008L20Rik, EFHA1)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLex 983-Micu2 was a gift from Vamsi Mootha (Addgene plasmid # 50372 ; http://n2t.net/addgene:50372 ; RRID:Addgene_50372)

  • For your References section:

    MICU2, a paralog of MICU1, resides within the mitochondrial uniporter complex to regulate calcium handling. Plovanich M, Bogorad RL, Sancak Y, Kamer KJ, Strittmatter L, Li AA, Girgis HS, Kuchimanchi S, De Groot J, Speciner L, Taneja N, Oshea J, Koteliansky V, Mootha VK. PLoS One. 2013;8(2):e55785. doi: 10.1371/journal.pone.0055785. Epub 2013 Feb 7. 10.1371/journal.pone.0055785 PubMed 23409044
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