pcDNAM-human eIF2C1-WT-Myc
(Plasmid
#50360)
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PurposeExpression of human eIF2C1 in mamalian cells
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50360 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown (a derivative of pVP22/Myc-His)
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeIF2C1
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Alt nameargonaute RISC catalytic component 1
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Alt nameargonaute 1
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Alt nameAGO1
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SpeciesH. sapiens (human)
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GenBank IDNM_012199
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Entrez GeneAGO1 (a.k.a. EIF2C, EIF2C1, GERP95, NEDLBAS, Q99, hAgo1)
- Promoter CMV
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Tag
/ Fusion Protein
- myc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNAM-human eIF2C1-WT-Myc was a gift from Kumiko Ui-Tei (Addgene plasmid # 50360 ; http://n2t.net/addgene:50360 ; RRID:Addgene_50360) -
For your References section:
Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K. Curr Biol. 2003 Jan 8;13(1):41-6. 10.1016/S0960-9822(02)01394-5 PubMed 12526743
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/64/14/774a90ca-52dd-11e3-a374-000c29055998.png.940x940_q85_autocrop.png)