Skip to main content
Addgene

pBluescript-mouse Dicer
(Plasmid #50357)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript II KS-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 5609
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dicer1
  • Alt name
    Dicer
  • Species
    M. musculus (mouse)
  • GenBank ID
    AB081470
  • Entrez Gene
    Dicer1 (a.k.a. 1110006F08Rik, D12Ertd7e, aviD, mKIAA0928)
  • Promoter T3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript-mouse Dicer was a gift from Kumiko Ui-Tei (Addgene plasmid # 50357 ; http://n2t.net/addgene:50357 ; RRID:Addgene_50357)

  • For your References section:

    Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K. Curr Biol. 2003 Jan 8;13(1):41-6. 10.1016/S0960-9822(02)01394-5 PubMed 12526743
Related items:
Commonly requested with: