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Addgene

pBluescript-mouse eIF2C4
(Plasmid #50356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50356 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 5609
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eIF2C4
  • Alt name
    argonaute4
  • Species
    M. musculus (mouse)
  • GenBank ID
    AB081474
  • Entrez Gene
    Ago4 (a.k.a. 5730550L01Rik, AI481660, Eif2c, Eif2c4)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing has found a A128V mutation relative to the depositor sequence, it is a known isoform.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript-mouse eIF2C4 was a gift from Kumiko Ui-Tei (Addgene plasmid # 50356 ; http://n2t.net/addgene:50356 ; RRID:Addgene_50356)

  • For your References section:

    Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K. Curr Biol. 2003 Jan 8;13(1):41-6. 10.1016/S0960-9822(02)01394-5 PubMed 12526743
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