pET28a-TRBP
(Plasmid #50351)

• Purpose: bacterial expression of TRBP
• Depositing Lab: Kumiko Ui-Tei
• Publication: Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 10.1371/journal.pone.0063434. Print 2013.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TRBP
• Alt name: TARBP2
• Alt name: TAR (HIV-1) RNA binding protein 2
• Species: H. sapiens (human)
• Entrez Gene: TARBP2 (a.k.a. LOQS, TRBP, TRBP1, TRBP2)
• Promoter: T7
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Fragment encoding a full length human TRBP was amplified using a cDNA mixture of total RNA extracted from human HeLa cells by PCR with primers containing restriction enzyme sites of HindIII and NotI. The amplified fragment was digested with the same enzyme sites and cloned into pcDNA3.1-myc/His A vector (Invitrogen). C-terminal myc-tagged TRBP fragment was amplified from pcDNA3.1-TRBP using primer with restriction enzyme sites NheI and HindIII. The amplified fragments were digested with same enzyme sites and cloned into pET-28a vector (Novagen).