pLYS1-MICU1-Flag
(Plasmid #50058)

• Purpose: Expression of human MICU1 with Flag tag in mammalian cells
• Depositing Lab: Vamsi Mootha
• Publication: Sancak et al Science. 2013 Nov 14.

Backbone

• Vector backbone: pLYS1
• Backbone size w/o insert (bp): 7393
• Total vector size (bp): 8824
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MICU1 (isoform 2)
• Species: H. sapiens (human)
• Insert Size (bp): 1431
• Mutation: *
• GenBank ID: NM_001195518
• Entrez Gene: MICU1 (a.k.a. CALC, CBARA1, EFHA3, MPXPS, ara CALC)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: Xma1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: custom

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*There are some silent mutations at the 3' end of the insert. These were used for resistance to a specific shRNA and will not affect function.